Abstract
A meta-QTL analysis was conducted in Indian mustard to identify robust and stable meta-QTLs (MQTLs) by utilizing 1504 available QTLs, which included 891 QTLs for yield-related traits and 613 QTLs for quality traits. For yield-related traits, a total of 57 MQTLs (YRTs_MQTLs) were uncovered from the clustering of 560 projected QTLs, which had a 4.18-fold smaller confidence interval (CI) than that of the initial QTLs, whereas, for quality traits, as many as 51 MQTLs (Quality_MQTLs) were derived from 324 projected QTLs, which had a 2.65-fold smaller CI than that of the initial QTLs. Sixteen YRTs_MQTLs were observed to share chromosomal positions with 16 Quality_MQTLs. Moreover, four most promising YRTs_MQTLs and eight Quality-MQTLs were also selected and recommended for use in breeding programs. Four of these selected MQTLs were also validated with significant SNPs that were identified in previously published genome-wide association studies. Further, in silico functional analysis of some promising MQTLs allowed the detection of as many as 1435 genes, which also involved 15 high-confidence candidate genes (CGs) for yield-related traits and 46 high-confidence CGs for quality traits. After validation, the identified CGs can also be exploited to model the plant architecture and to improve quality traits through marker-assisted breeding, genetic engineering, and genome editing approaches.
Subject
Agronomy and Crop Science
Reference87 articles.
1. Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization;Nagaharu;Jpn. J. Bot.,1935
2. Molecular markers for yield components in Brassica juncea – do these assist in breeding for high seed yield?
3. Chemical composition of Brassica juncea seeds used to prepare the mustard of Dijon;Vangheesdaele;Rev. Fr. Corps Gras.,1980
4. Hundred years of rapeseed-mustard breeding in India: Accomplishments and future strategies;Chauhan;Indian J. Agric. Sci.,2011
5. Breeding for Improved Oil Quality in Brassica Oilseed Species
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