Integrated Transcriptome and Metabolome Analysis Reveal That Exogenous Gibberellin Application Regulates Lignin Synthesis in Ramie

Abstract

Gibberellin regulates plant growth, development, and metabolic processes. However, the underlying mechanism of the substantial effect of gibberellin on stem height and secondary metabolites in forage ramie is unclear. Therefore, this study combined transcriptomic and metabolomics analyses to identify the mechanisms regulating growth and secondary metabolite contents in forage ramie following exogenous gibberellin application. Exogenous gibberellin application significantly reduced the lignin content in the leaves but not in the stems. At the same time, gibberellin significantly increased the total flavonoid and chlorogenic acid contents in both the stems and leaves. In addition, 293 differentially expressed genes (DEGs) and 68 differentially expressed metabolites (DEMs) were identified in the leaves. In the stems, 128 DEGs and 41 DEMs were identified. The DEGs PER42, FLS, CYP75A, and PNC1 were up-regulated in the leaves, affecting phenylpropane metabolism. The joint analysis of the DEMs and DEGs revealed that the changes in the DEGs and DEMs in the leaves and stems improved the substrate efficiency in the phenol propane pathway and inhibited lignin synthesis in plants, thus shifting to flavonoid pathway synthesis. In conclusion, gibberellin treatment effectively reduces the lignin content in forage ramie while increasing the flavonoid and chlorogenic acid contents. These findings provide empirical and practical guidance for breeding for forage quality in ramie and the improvement and cultivation control of forage ramie.