Transcriptome-Wide Analysis of Core Transcription Factors Associated with Defense Responses in Autotetraploid versus Diploid Rice under Saline Stress and Recovery
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Published:2023-11-05
Issue:21
Volume:24
Page:15982
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Wang Yingkai1,
Meng Weilong1,
Ye Yan1,
Yu Xinfang1,
Chen Haiyuan1,
Liu Yuchen1,
Xu Minghong1,
Wang Ningning12,
Qi Fan1,
Lan Yujie1,
Xu Yan1,
Ma Jian12,
Zhang Chunying12
Affiliation:
1. Faculty of Agronomy, Jilin Agricultural University, Changchun 130000, China
2. Jilin Provincial Laboratory of Crop Germplasm Resources, Changchun 130000, China
Abstract
Saline stress is a major abiotic stress that inhibits plant growth and yields worldwide. The plant transcription factor (TF) family plays an important role in converting abiotic stress signals into gene expression changes. In this study, a transcriptome-based comparative analysis was performed to investigate the global gene expression of all the TFs in diploid and autotetraploid rice during the early stage of NaCl stress and recovery period. The phenotypic data indicated that the tetraploid rice exhibited a superior salt-tolerant ability compared to the diploid rice. A total of 55 TF families were co-expressed in the tetraploid and diploid rice, and the cumulative number of TF-expressed genes was relatively higher in the diploid rice than in the tetraploid rice at all time points. Unlike the diploid rice, the overall gene expression levels of the tetraploid rice were comparable to the control during recovery. The number of differentially expressed TFs (DE-TFs) in the tetraploid rice decreased after recovery, whereas it increased to a large extent in the diploid rice. GO and KEGG pathway enrichment analysis of the DE-TFs discovered the early switching of the ABA-activated signaling pathway and specific circadian rhythm in the tetraploid rice. Combining the PPI network and heatmap analysis, some core DE-TFs were found that may have potential roles to play in tetraploid salt tolerance. This study will pave the way for elucidating the complex network regulatory mechanisms of salt tolerance in tetraploid rice.
Funder
Jilin Provincial Research Foundation for Technologies Research of China
National Rice Industry Cluster Project, Doctoral Research Startup Funds
National & Provincial College Student Innovation and Entrepreneurship Training Projects recommended by Jilin Agricultural University in 2023
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis