Abstract
The USDA-FSIS has zero tolerance for E. coli O157:H7 in raw ground beef. Currently, FSIS collects samples from beef processing facilities and ships them overnight to regional testing laboratories. Pathogen detection requires robust methods that employ an initial 15–24 h culture enrichment. This study assessed the potential of using the ΦV10nluc phage-based luminescence detection assay during enrichment while the sample is in transit. Parameters including phage concentrations, temperature, and media-to-sample ratios were evaluated. Results in liquid media showed that 1.73× 103 pfu/mL of ΦV10nluc was able to detect 2 CFU in 10 h. The detection of E. coli O157:H7 was further evaluated in kinetic studies using ratios of 1:3, 1:2, and 1:1 ground beef sample to enrichment media, yielding positive results for as little as 2–3 CFU in 325 g ground beef in about 15 h at 37 °C. These results suggest that this approach is feasible, allowing the detection of a presumptive positive upon arrival of the sample to the testing lab. As the current cargo hold controlled temperature is required to be 15–25 °C, the need for elevated temperature should be easily addressed. If successful, this approach could be expanded to other pathogens and foods.
Funder
U.S. Department of Agriculture, Agricultural Research Service, Food Safety program in-house projects
Purdue University Center for Food Safety Engineering
USDA National Institute of Food and Agriculture
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science
Reference29 articles.
1. Foodborne Illness Acquired in the United States—Major Pathogens;Emerg. Infect. Dis.,2011
2. Molecular Mechanisms of Escherichia coli Pathogenicity;Nat. Rev. Microbiol.,2010
3. (2022, September 15). Centers of Disease Control and Prevention. E. coli (Escherichia coli). U.S. Department of Health and Human Services, Available online: https://www.cdc.gov/ecoli/general/index.html.
4. Hemolytic Uremic syndrome;Sem. Immunopathol.,2014
5. EFSA (2014). The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 2012. EFSA J., 12, 3547.
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