Author:
Jiang Shunyi,Song Danni,Zhao Honghui,Wang Fuqi,Su Xin,Zhang Xinyang,Zhao Xu
Abstract
Hyperuricemia (HUA) is a metabolic condition caused by excessive production or low excretion of uric acid (UA) in the body. Xanthine oxidase (XOD) is the key enzyme in the process of metabolism purines to generate UA. In this study, the in vitro inhibitory effect of water extract of the flower bud of Sophora japonica (WESJ) on XOD was investigated by ultraviolet spectrophotometry. A mice model of HUA was constructed to explore the effect of WESJ on UA levels and the mechanism of action on renal function. Based on Box–Behnken design, the optimal extraction process of WESJ was determined to extract Sophora japonica twice with 8 times of water, 0.5 h each time. Pharmacological results showed that low, medium, and high doses of WESJ (200, 400, 600 mg/kg) could significantly reduce serum UA level, inhibit the activity of XOD in blood and liver, and have a protective effect on kidney damage caused by high UA. Through UPLC-Q-TOF-MS/MS analysis, 214 compounds were identified in WESJ, including flavonoids, polyphenols, triterpenoids, organic acids, and others. The rat serum of WESJ was analyzed, and 23 prototype components entering the blood were identified, including 15 flavonoids and polyphenols, which may be the main bioactive components. In conclusion, flavonoids and polyphenols in WESJ may reduce the level of UA and alleviate kidney damage by inhibiting the activity of XOD. WESJ is expected to be used as a plant-based food and dietary supplement for the treatment of HUA.
Funder
Young and Middle Aged Teachers’ Career Development Support Project of Shenyang Pharmaceutical University
Innovation and Entrepreneurship Training Program for College Students of Shenyang Pharmaceutical University
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science
Reference35 articles.
1. Mutations associated with functional disorder of xanthine oxidoreductase and hereditary xanthinuria in humans;Int. J. Mol. Sci.,2012
2. Chlorogenic acid supplementation ameliorates hyperuricemia, relieves renal inflammation, and modulates intestinal homeostasis;Food Funct.,2021
3. Hong, F., Zheng, A., Xu, P., Wang, J., Xue, T., Dai, S., Pan, S., Guo, Y., Xie, X., and Li, L. (2020). High-Protein Diet Induces Hyperuricemia in a New Animal Model for Studying Human Gout. Int. J. Mol. Sci., 21.
4. Association between three non-insulin-based indexes of insulin resistance and hyperuricemia;Clin. Rheumatol.,2019
5. Hyperuricemia complicated with acute kidney injury is associated with adverse outcomes in patients with severely decompensated acute heart failure;Int. J. Cardiol. Heart Vasc.,2019
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献