Study of the Effect of Cell Prestress on the Cell Membrane Penetration Behavior by Atomic Force Microscopy

Author:

Zhang Guocheng1,Chang Yufang2,Fan Na3,Yan Bin1,Li Xianmeng1,Yang Zihan1,Yu Zhenyang1

Affiliation:

1. Department of Mechanical Engineering, Anyang Institute of Technology, Yellow River Avenue West, Anyang 455000, China

2. School of Accountancy, Anyang Institute of Technology, Yellow River Avenue West, Anyang 455000, China

3. School of Mechanical and Electrical Engineering, University of Electronic Science and Technology of China, 2006 Xiyuan Avenue, Hi-Tech West District, Chengdu 611731, China

Abstract

In recent years, atomic force microscopes have been used for cell transfection because of their high-precision micro-indentation mode; however, the insertion efficiency of the tip of AFM into cells is extremely low. In this study, NIH3T3 mouse fibroblast cells cultured on a flexible dish with micro-groove patterns were subjected to various substrate strains at 5%, 10%, 15%, and 20%. It was found that the cell stiffness depends on the prestress of the cell membrane, and that the insertion rate of AFM tips into the cell membrane is proportional to the stiffness through the AFM indentation experiment. The finite element analysis proves that prestress increases the bending stiffness of the cytoskeleton, allowing it to better support the cell membrane, which realizes the stress concentration in the contact area between the AFM tip and the cell membrane. The results indicate that the prestress contributes to the mechanical properties of the cell and suggest that the insertion efficiency could be greatly improved with an increase of the prestress of the cell membrane.

Funder

National Natural Science Foundation of China

Science and Technology of Anyang

Key Science and Technology Plan Project of Henan province of China

Key Scientific Research Projects of Universities in Henan

Startup Foundation for doctor in Anyang Institute of Technology

Publisher

MDPI AG

Subject

Electrical and Electronic Engineering,Mechanical Engineering,Control and Systems Engineering

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