Detection of IgA and IgG Antibodies against the Structural Proteins of SARS-CoV-2 in Breast Milk and Serum Samples Derived from Breastfeeding Mothers
Author:
Cortés-Sarabia Karen1ORCID, Guzman-Silva Vianey1, Martinez-Pacheco Karla Montserrat1, Meza-Hernández Jesús Alberto1, Luna-Pineda Víctor Manuel2ORCID, Leyva-Vázquez Marco Antonio3ORCID, Vences-Velázquez Amalia1, Beltrán-Anaya Fredy Omar3ORCID, Del Moral-Hernández Oscar4ORCID, Illades-Aguiar Berenice2ORCID
Affiliation:
1. Laboratorio de Inmunobiología y Diagnóstico Molecular, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo de los Bravo 39086, Mexico 2. Unidad de Investigación en Inmunología y Proteómica, Laboratorio de Investigación en COVID-19, Hospital Infantil de México “Federico Gómez”, Mexico City 06720, Mexico 3. Laboratorio de Biomedicina Molecular, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo de los Bravo 39086, Mexico 4. Laboratorio de Virología, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo de los Bravo 39086, Mexico
Abstract
Background: COVID-19 vaccination or natural infection is associated with the development of immunity. The search of IgA and IgG antibodies against all the structural proteins (spike, nucleocapsid, membrane, and envelope) of SARS-CoV-2 in breastfeeding mothers is associated with immunity that can help the newborn avoid development of the infection. Methods: In this study, we analyzed 30 breastfeeding women that provided samples of breast milk and serum and evaluated the presence of IgA, total IgG, and subclasses against the structural proteins of SARS-CoV-2. Results: We reported a high seroprevalence to IgA (76.67–100%) and negativity to IgG against all analyzed proteins in breast milk. Seroprevalence in serum samples was around 10–36.67% to IgA and 23.3–60% to IgG. Finally, we detected the presence of the subclasses IgG1, IgG2, and IgG4 against all the structural proteins of SARS-CoV-2. Conclusions: This work provides evidence of the presence of IgA and IgG antibodies against the four structural proteins of SARS-CoV-2 in breast milk and serum samples derived from breastfeeding women, which can confer immunity to the newborn.
Subject
Virology,Infectious Diseases
Reference43 articles.
1. The Incubation Period of Coronavirus Disease 2019 (COVID-19) From Publicly Reported Confirmed Cases: Estimation and Application;Lauer;Ann. Intern. Med.,2020 2. Rando, H.M., MacLean, A.L., Lee, A.J., Lordan, R., Ray, S., Bansal, V., and Greene, C.S. (2021). Pathogenesis, Symptomatology, and Transmission of SARS-CoV-2 through Analysis of Viral Genomics and Structure. arXiv. 3. Indirect Virus Transmission in Cluster of COVID-19 Cases, Wenzhou, China, 2020;Cai;Emerg Infect. Dis.,2020 4. Cascella, M., Rajnik, M., Aleem, A., Dulebohn, S.C., and Di Napoli, R. (2023, February 15). Features, Evaluation, and Treatment of Coronavirus (COVID-19), Available online: http://www.ncbi.nlm.nih.gov/books/NBK554776/. 5. Genome-Wide Identification and Characterization of Point Mutations in the SARS-CoV-2 Genome;Kim;Osong. Public Health Res. Perspect.,2020
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