Combined Effects of Defatted Hydrolyzed Collagen from Salmon Skin and Vitamin C on Proliferation and Migration of Human Fibroblast Cell

Abstract

Skin injury causes fibroblast dysfunction and lowers collagen production. Safe, functional ingredients such as vitamin C (Vit C) and fish hydrolyzed collagen (HC) have been used to alleviate this problem. Defatted HC from salmon (Oncorhynchus nerka) skin could be a potential functional ingredient with skin nourishment activity. This study aimed to investigate the combined effects of HC and Vit C on the proliferation and migration of human dermal fibroblast (HDF). Molecular weight ranging from 102 Da to 10,175 Da and high imino acid content were found in HC. HC (0–800 µg/mL) or vitamin C (Vit C) (0.01–100 µg/mL) was applied for HDF treatment. Higher cell proliferation was found by adding HC at 50 µg/mL or Vit C at 0.01 µg/mL compared to the control and those treated with both compounds at other levels (p < 0.05). Cells treated with HC (50 µg/mL) combined with Vit C (0.01 µg/mL) (HC+Vit C) showed higher proliferation, migration, and lamellipodia formation of HDF cells than those treated with HC or Vit C alone. Moreover, all the samples tested could stimulate the proliferation and migration of HDF cells via FAK/Akt and ERK/p38 MAPK signaling pathways. Thus, HC combined with Vit C could be a promising functional ingredient for skin nourishment and would healing.