Effect of Dietary Moringa oleifera Leaves Nanoparticles on Growth Performance, Physiological, Immunological Responses, and Liver Antioxidant Biomarkers in Nile tilapia (Oreochromis niloticus) against Zinc Oxide Nanoparticles Toxicity

Abstract

The current study addresses the influence of Moringa oleifera leaves nanoparticles (MO-NPs) on growth, biochemical, immunological, and hepatic antioxidant alterations induced by zinc oxide nanoparticles toxicity in Nile tilapia (O. niloticus). Fish (N = 180) were divided into four groups with replicates. The first one was set as a control group and the second group was fed an MO-NPs-enriched diet (2.5 g/kg diet). The third group was exposed to 8 mg/L ZnO-NPs, while the forth group was exposed to 8 mg/L ZnO-NPs and fed on MO-NPs (2.5 g/kg diet) for 2 months. Exposure of O. niloticus to 8 mg/L ZnO-NPs induced the following consequences: a sharp decrease in the growth parameters; a marked increment in the biochemical biomarkers (glucose, cortisol, and liver enzymes (ALT, AST, ALP); a significant increase in serum renal products, urea and creatinine, cholesterol, and LDH levels. Nonetheless, the dietary MO-NPs supplementation for 2 months significantly alleviated the ZnO-NPs toxicity and significantly enhanced the growth indices, plus normalizing the physio-biochemical levels in the exposed group to ZnO-NPs toxicity to reach the levels of the control group. The MO-NPs markedly improved hepatic antioxidant biomarkers, MDA, and TAC, while, decreasing SOD, CAT, and GSH levels to be near the control values. Moreover, supplemented fish in MO-NPs (2.5 g/kg diet) and exposed to ZnO-NPs provided a remarkable increase in the immune profile (respiratory burst (RB) activity, lysozyme, and total immunoglobulins (IgM)) compared to the ZnO-NPs-intoxicated group. Based on the findings of the study, the exposed O. niloticus to ZnO-NPs were immune-antioxidant-depressed, besides showing growth retardation, and physio-biochemical alterations. On the other hand, a supplemented diet with MO-NPs is a novel approach to ameliorate ZnO-NPs toxicity for sustaining aquaculture and correspondingly protecting human health.