Different Methods for Seminal Plasma Removal and Sperm Selection on the Quality and Fertility of Collared Peccary Sperm

Author:

Santos Maria V. O.1ORCID,Silva Andréia M.2ORCID,Aquino Leonardo V. C.1ORCID,Oliveira Lhara R. M.1ORCID,Moreira Samara S. J.2ORCID,Oliveira Moacir F.3ORCID,Silva Alexandre R.2ORCID,Pereira Alexsandra F.1ORCID

Affiliation:

1. Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Mossoro 59625-900, RN, Brazil

2. Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid, Mossoro 59625-900, RN, Brazil

3. Laboratory of Applied Animal Morphophysiology, Federal Rural University of Semi-Arid, Mossoro 59625-900, RN, Brazil

Abstract

Methods for seminal plasma (SP) removal and the selection of collared peccary sperm for fertilization were compared. The experiments evaluated the following: the (I) impact of centrifugation for SP removal before swim-up for sperm selection and (II) a comparison of different Percoll® gradient densities (PG 45–90% and PG 35–70%). Non-selected sperm served as the control. Sperm quality was assessed based on motility patterns, morphology, membrane functional integrity, viability, reactive oxygen species (ROS), glutathione (GSH), and DNA integrity. Subsequently, the most successful group in the previous experiment and washing by centrifugation (WC) were compared for motility patterns and fertilization using pig oocytes. Swim-up decreased motility and enhanced ROS compared to the control. Centrifugation before swim-up harmed integrity and viability compared to the control. PG 45–90% (96.8 vs. 69.7 vs. 40.7 µm/s) allowed for a better velocity average pathway (VAP), a better velocity straight line, and better linearity (LIN) than those of the control and PG 35–70% (88.4 vs. 56.0 vs. 27.3 µm/s). Thus, PG 45–90% was used for fertilization. PG 45–90% obtained a higher VAP, a higher amplitude of the lateral head, straightness, and higher LIN than those of the control and WC. Cleavage (25.2–26.3%) and morula (8.1–10.5%) rates did not differ between the groups. Therefore, PG 45–90% and WC were efficient in isolating collared peccary sperm capable of fertilizing pig oocytes.

Funder

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil

National Council for Scientific Development

Publisher

MDPI AG

Subject

General Veterinary,Animal Science and Zoology

Reference56 articles.

1. Gongora, J., Reyna-Hurtado, R., Beck, H., Taber, A., Altrichter, M., and Keuroghlian, A. (2011). The IUCN Red List of Threatened Species, IUCN.

2. Avaliação do risco de extinção do cateto Pecari tajacu Linnaeus, 1758, no Brasil;Desbiez;Bio Brasil,2012

3. Abundance, herd size, activity pattern and occupancy of ungulates in Southeastern Mexico;Anim. Biol.,2016

4. Coexistence of jaguar (Panthera onca) and puma (Puma concolor) in a mosaic landscape in the Venezuelan llanos;Scognamillo;J. Zool.,2003

5. Use of wildlife as an alternative protein source: Collared peccary meat;Morais;Meat Sci.,2022

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3