Development of Clindamycin-Releasing Polyvinyl Alcohol Hydrogel with Self-Healing Property for the Effective Treatment of Biofilm-Infected Wounds
Author:
Alifah Nur1, Palungan Juliana1, Ardayanti Kadek1, Ullah Muneeb2, Nurkhasanah Andi Nokhaidah1, Mustopa Apon Zaenal3, Lallo Subehan1ORCID, Agustina Rina1, Yoo Jin-Wook2ORCID, Hasan Nurhasni1ORCID
Affiliation:
1. Faculty of Pharmacy, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar 90245, Indonesia 2. College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea 3. Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor 16911, Indonesia
Abstract
Self-healing hydrogels have good mechanical strength, can endure greater external force, and have the ability to heal independently, resulting in a strong bond between the wound and the material. Bacterial biofilm infections are life-threatening. Clindamycin (Cly) can be produced in the form of a self-healing hydrogel preparation. It is noteworthy that the antibacterial self-healing hydrogels show great promise as a wound dressing for bacterial biofilm infection. In this study, we developed a polyvinyl alcohol/borax (PVA/B) self-healing hydrogel wound dressing that releases Cly. Four ratios of PVA, B, and Cly were used to make self-healing hydrogels: F1 (4%:0.8%:1%), F2 (4%:1.2%:1%), F3 (1.6%:1%), and F4 (4%:1.6%:0). The results showed that F4 had the best physicochemical properties, including a self-healing duration of 11.81 ± 0.34 min, swelling ratio of 85.99 ± 0.12%, pH value of 7.63 ± 0.32, and drug loading of 98.34 ± 11.47%. The B–O–C cross-linking between PVA and borax caused self-healing, according to FTIR spectra. The F4 formula had a more equal pore structure in the SEM image. The PVA/B-Cly self-healing hydrogel remained stable at 6 ± 2 °C for 28 days throughout the stability test. The Korsmeyer–Peppas model released Cly by Fickian diffusion. In biofilm-infected mouse wounds, PVA/B-Cly enhanced wound healing and re-epithelialization. Our results indicate that the PVA/B-Cly produced in this work has reliable physicochemical properties for biofilm-infected wound therapy.
Funder
Indonesia Endowment Funds for Education
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