Effects of NaCl Stress on the Growth, Physiological Characteristics and Anatomical Structures of Populus talassica × Populus euphratica Seedlings

Abstract

In order to elucidate the salt tolerance mechanism of Populus talassica × Populus euphratica, the growth, physiology and anatomical characteristics of P. talassica × P. euphratica were studied under different concentrations of NaCl-stress treatments. In this study, the annual seedlings of Populus talassica × Populus euphratica were used as the test material in a field potted control experiment. The basic salt content of the culture soil was the control (CK), and two NaCl treatments of 200 mmol/L and 400 mmol/L were established. The pot experiment showed that: (1) Compared with CK, the 200 mmol/L NaCl-stress treatment significantly increased the growth parameters of P. talassica × P. euphratica, such as leaf area, plant height, ground diameter, biomass, root length, root surface area, root fork number and root-shoot ratio. However, compared with CK, the 400 mmol/L NaCl-stress treatment significantly reduced most growth parameters. (2) The 200 and 400 mmol/L NaCl-stress treatments significantly decreased various physiological parameters such as relative water content (RWC), chlorophyll content, water potential, stomatal opening and photosynthetic parameters and increased the accumulation of MDA and Pro compared with CK. The 200 mmol/L NaCl-stress treatment significantly increased the activity of antioxidant enzymes, and the 400 mmol/L NaCl-stress treatment significantly decreased the activity of antioxidant enzymes. (3) Compared with CK, 200 and 400 mmol/L NaCl-stress treatments significantly improved the leaf palisade tissue thickness and palisade-to-sea ratio, as well as the stem xylem and stem phloem thickness and pith diameter, and significantly increased the root xylem thickness, root phloem thickness, and root cross-cutting diameter of P. talassica × P. euphratica. The growth, physiological characteristics and anatomical characteristics of P. talassica × P. euphratica under NaCl-stress treatments showed that it had good salt tolerance and adaptability, and the 200 mmol/L NaCl-stress treatment promoted the growth of P. talassica × P. euphratica to a certain extent. This study provided a theoretical basis for the study of the salt-tolerant mechanism of P. talassica × P. euphratica.