Enzymes Immobilized into Starch- and Gelatin-Based Hydrogels: Properties and Application in Inhibition Assay

Author:

Esimbekova Elena N.12ORCID,Torgashina Irina G.1ORCID,Nemtseva Elena V.12ORCID,Kratasyuk Valentina A.12

Affiliation:

1. Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia

2. Laboratory of Photobiology, Institute of Biophysics of Siberian Branch of Russian Academy of Science, 660036 Krasnoyarsk, Russia

Abstract

The present work is a review of the research on using hydrogels based on natural biodegradable polymers, starch, and gelatin for enzyme immobilization. This review addresses the main properties of starch and gelatin that make them promising materials in biotechnology for producing enzyme preparations stable during use and storage and insensitive to chemical and physical impacts. The authors summarize their achievements in developing the preparations of enzymes immobilized in starch and gelatin gels and assess their activity, stability, and sensitivity for use as biorecognition elements of enzyme inhibition-based biosensors.

Funder

Russian Science Foundation

Krasnoyarsk Regional Fund of Science

Publisher

MDPI AG

Subject

Electrical and Electronic Engineering,Mechanical Engineering,Control and Systems Engineering

Reference108 articles.

1. Use of Enzymes in Dairy Industry: A Review of Current Progress;Khan;Arch. Razi Inst.,2020

2. An overview of Bacillus proteases: From production to application;Contesini;Crit. Rev. Biotechnol.,2018

3. Current Trends in Protein Engineering: Updates and Progress;Sinha;Curr. Protein Pept. Sci.,2019

4. Protein engineering approaches to chemical biotechnology;Chen;Curr. Opin. Biotechnol.,2016

5. Biosensors based on enzyme inhibition;Gu;Biosensors Based on Aptamers and Enzymes,2014

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