Affiliation:
1. Independent Researcher, New Delhi, India
2. Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak-124001, Haryana, India
Abstract
Proteins are one of the most important and resourceful biomolecules that find applications
in health, industry, medicine, research, and biotechnology. Given its tremendous relevance, protein
engineering has emerged as significant biotechnological intervention in this area. Strategic utilization
of protein engineering methods and approaches has enabled better enzymatic properties, better stability,
increased catalytic activity and most importantly, interesting and wide range applicability of proteins.
In fact, the commercialization of engineered proteins have manifested in economically beneficial
and viable solutions for industry and healthcare sector. Protein engineering has also evolved to become
a powerful tool contributing significantly to the developments in both synthetic biology and metabolic
engineering. The present review revisits the current trends in protein engineering approaches such as
rational design, directed evolution, de novo design, computational approaches etc. and encompasses
the recent progresses made in this field over the last few years. The review also throws light on advanced
or futuristic protein engineering aspects, which are being explored for design and development
of novel proteins with improved properties or advanced applications.
Funder
Department of Biotechnology, Government of India
Department of Science and Technology, New Delhi, Govt. of India
Publisher
Bentham Science Publishers Ltd.
Subject
Cell Biology,Molecular Biology,Biochemistry,General Medicine
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