Transcriptome Changes of Hematopoietic Stem and Progenitor Cells in the Peripheral Blood of COVID-19 Patients by scRNA-seq
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Published:2023-06-29
Issue:13
Volume:24
Page:10878
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Zhang Jinfu1ORCID,
Shu Xin1,
Deng Rong1,
Yang Zihao1,
Shu Manling1ORCID,
Ou Xiangying1,
Zhang Xuan1,
Wu Zhenyu1,
Zeng Huihong2,
Shao Lijian1
Affiliation:
1. Department of Occupational Health and Toxicology, School of Public Health, Nanchang University, Nanchang 330006, China
2. Department of Histology and Embryology, School of Basic Medicine, Nanchang University, Nanchang 330006, China
Abstract
Coronavirus disease 2019 (COVID-19) threatens public health all over the world. It is well-accepted that the immune cells in peripheral blood are widely involved in the pathological process of COVID-19. However, hematopoietic stem and progenitor cells (HSPCs), as the main source of peripheral immune cells, have not been well studied during COVID-19 infection. We comprehensively revealed the transcriptome changes of peripheral blood HSPCs after COVID-19 infection and vaccination by single-cell RNA-seq. Compared with healthy individuals, the proportion of HSPCs in COVID-19 patients significantly increased. The increase in the proportion of HSPCs might be partly attributed to the enhancement of the HSPCs proliferation upon COVID-19 infection. However, the stemness damage of HSPCs is reflected by the decrease of differentiation signal, which can be used as a potential specific indicator of the severity and duration of COVID-19 infection. Type I interferon (IFN-I) and translation signals in HSPCs were mostly activated and inhibited after COVID-19 infection, respectively. In addition, the response of COVID-19 vaccination to the body is mild, while the secondary vaccination strengthens the immune response of primary vaccination. In conclusion, our study provides new insights into understanding the immune mechanism of COVID-19 infection.
Funder
the National Natural Science Foundation of China
Jiangxi Provincial Natural Science Foundation
the Recruitment Program of Global Experts in Jiangxi Province
Interdisciplinary Innovation Fund of Natural Science in Nanchang University
Graduate Innovation Special Fund of Jiangxi Province
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis