Functional Study of cAMP-Dependent Protein Kinase A in Penicillium oxalicum
-
Published:2023-12-16
Issue:12
Volume:9
Page:1203
-
ISSN:2309-608X
-
Container-title:Journal of Fungi
-
language:en
-
Short-container-title:JoF
Author:
Sun Qiuyan1, Xu Gen1, Li Xiaobei1, Li Shuai1, Jia Zhilei1, Yan Mengdi1, Chen Wenchao1, Shi Zhimin1, Li Zhonghai1ORCID, Chen Mei1
Affiliation:
1. State Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Shandong Provincial Key Laboratory of Microbial Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
Abstract
Signaling pathways play a crucial role in regulating cellulase production. The pathway mediated by signaling proteins plays a crucial role in understanding how cellulase expression is regulated. In this study, using affinity purification of ClrB, we have identified sixteen proteins that potentially interact with ClrB. One of the proteins, the catalytic subunit of cAMP-dependent protein kinase A (PoPKA-C), is an important component of the cAMP/PKA signaling pathway. Knocking out PoPKA-C resulted in significant decreases in the growth, glucose utilization, and cellulose hydrolysis ability of the mutant strain. Furthermore, the cellulase activity and gene transcription levels were significantly reduced in the ΔPoPKA-C mutant, while the expression activity of CreA, a transcriptional regulator of carbon metabolism repression, was notably increased. Additionally, deletion of PoPKA-C also led to earlier timing of conidia production. The expression levels of key transcription factor genes stuA and brlA, which are involved in the production of the conidia, showed significant enhancement in the ΔPoPKA-C mutant. These findings highlight the involvement of PoPKA-C in mycelial development, conidiation, and the regulation of cellulase expression. The functional analysis of PoPKA-C provides insights into the mechanism of the cAMP/PKA signaling pathway in cellulase expression in filamentous fungi and has significant implications for the development of high-yielding cellulase strains.
Funder
Natural Science Foundation of Shandong Province Qilu University of Technology scientific research project of Qilu University of Technology Shandong and Chongqing Science and Technology Cooperation Project
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)
Reference45 articles.
1. Li, Z.H., Yao, G.S., Wu, R.M., Gao, L.W., Kan, Q.B., Liu, M., Yang, P., Liu, G.D., Qin, Y.Q., and Song, X. (2015). Synergistic and Dose-Controlled Regulation of Cellulase Gene Expression in Penicillium oxalicum. PLoS Genet., 11. 2. The transcription factor ACE3 controls cellulase activities and lactose metabolism via two additional regulators in the fungus Trichoderma reesei;Zhang;J. Biol. Chem.,2019 3. Jia, Z., Yan, M., Li, X., Sun, Q., Xu, G., Li, S., Chen, W., Shi, Z., Li, Z., and Chen, M. (2023). Phosducin-like protein PoPlp1 impacts cellulase and amylase expression and development in Penicillium oxalicum via the G protein-cAMP signaling pathway. Front. Microbiol., 14. 4. G protein-cAMP signaling pathway mediated by PGA3 plays different roles in regulating the expressions of amylases and cellulases in Penicillium decumbens;Hu;Fungal Genet. Biol.,2013 5. Transcriptional regulation of plant cell wall degradation by filamentous fungi;Aro;FEMS Microbiol. Rev.,2005
|
|