Validating and Developing Hyperspectral Indices for Tracing Leaf Chlorophyll Fluorescence Parameters under Varying Light Conditions

Abstract

Chlorophyll a fluorescence (ChlFa) parameters provide insight into the physiological and biochemical processes of plants and have been widely applied to monitor and evaluate the photochemical process and photosynthetic capacity of plants in a variety of environments. Recent advances in remote sensing provide new opportunities for the detection of ChlFa at large scales but demand further tremendous efforts. Among such efforts, application of the hyperspectral index is always possible, but the performance of hyperspectral indices in detecting ChlFa parameters under varying light conditions is much less investigated. The objective of this study is to investigate the performance of reported hyperspectral indices for tracking ChlFa parameters under different light conditions and to develop and evaluate novel spectral indices. Therefore, an experiment was conducted to simultaneously measure ChlFa parameters and spectral reflectance of sunlit and shaded leaves under varying light conditions, and 28 reported hyperspectral indices were examined for their performance in tracking the ChlFa parameters. Furthermore, we developed novel hyperspectral indices based on various spectral transformations. The results indicated that the maximum quantum efficiency of photosystem II (PSIImax), the cumulative quantum yield of photochemistry (ΦP), and the fraction of open reaction centers in photosystem II (qL) of sunlit leaves were significantly higher than those of shaded leaves, while the cumulative quantum yield of regulated thermal dissipation (ΦN) and fluorescence (ΦF) of shaded leaves was higher than that of sunlit leaves. Efficient tracing of ChlFa parameters could not be achieved from previously published spectral indices. In comparison, all ChlFa parameters were well quantified in shaded leaves when using novel hyperspectral indices, although the hyperspectral indices for tracing the non-photochemical quenching (NPQ) and ΦF were not stable, especially for sunlit leaves. Our findings justify the use of hyperspectral indices as a practical approach to estimating ChlFa parameters. However, caution should be used when using spectral indices to track ChlFa parameters based on the differences in sunlit and shaded leaves.