Effects of Plant Regulators on the Seed Germination and Antioxidant Enzyme Activity of Cotton under Compound Salt Stress
Author:
Dong Zhiduo12, Huang Jian13, Qi Tong134ORCID, Fu Qiuping2, Meng Ajing13, Fu Yanbo15
Affiliation:
1. Institute of Soil Fertilizer, Agricultural Water Saving, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China 2. College of Water Conservancy and Civil Engineering, Xinjiang Agricultural University, Urumqi 830052, China 3. Key Laboratory of Saline-Alkali Soil Improvement and Utilization (Saline-Alkali Land in Arid and Semi-Arid Regions), Ministry of Agriculture and Rural Affairs, Urumqi 830091, China 4. College of Land Science and Technology, China Agricultural University, Beijing 100193, China 5. National Soil Quality Aksu Observation Experimental Station, Aksu 843000, China
Abstract
Salinity stress significantly hampers cotton seed germination and seedling growth. Employing plant growth regulators stands out as an effective strategy to mitigate salt stress. In this study, we assessed the impact of varying concentrations of natural composite salt conditions (0%, 0.6%, and 1.2%) on cotton seed germination, seedling growth, and physiology. Additionally, we explored the effects of compound sodium nitrophenolate (CSN: 2 mg·L−1 and 10 mg·L−1), 24-epibrassinolide (EBR: 0.02 mg·L−1 and 0.1 mg·L−1), and gibberellic acid (GA: 60 mg·L−1 and 300 mg·L−1), against a control (CK: distilled water) group. The results indicate that with an increase in the composite salt concentration, the germination potential (GP) and germination rate (GR) of cotton seeds gradually decrease. Simultaneously, the fresh weight and root vitality of seedlings also correspondingly decrease, while the degree of membrane lipid peroxidation increases. Under high-salt (1.2%) conditions, soaking treatments with CSN and EBR significantly enhance both GP (45–59% and 55–64%) and GR (30–33% and 39–36%) compared to the CK. However, the GA treatment does not increase the GP and GR of cotton. Moreover, under high-salt (1.2%) conditions, CSN and EBR treatments result in the increased activities of superoxide dismutase (56–66% and 71–80%), peroxidase (20–24% and 37–51%), and catalase (26–32% and 35–46%). Consequently, cotton exhibits a relatively good tolerance to natural composite salts. Soaking treatments with CSN and EBR effectively improve cotton germination by enhancing antioxidant enzyme activities, thereby strengthening cotton’s tolerance to salt stress. These findings offer new insights for enhancing the salt tolerance of cotton.
Funder
Basic Scientific Research Business National Key Research and Development Program Major Special Project of Xinjiang Uygur Autonomous Region Xinjiang Province ‘Three Agriculture’ Key Talents Training Program
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
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