Morpho-Physiological Evaluation of Solanum betaceum Cav. In Vitro Cloned Plants: A Comparison of Different Micropropagation Methods
Author:
Correia Mariana1ORCID, Lopes Tércia1ORCID, Puga Ana Patrícia1, Pinto Glória2ORCID, Canhoto Jorge1ORCID, Correia Sandra13ORCID
Affiliation:
1. Center for Functional Ecology, TERRA Associate Laboratory, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal 2. Centre for Environmental and Marine Studies (CESAM), Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal 3. InnovPlantProtect CoLAB, Estrada de Gil Vaz, 7350-999 Elvas, Portugal
Abstract
Tamarillo (Solanum betaceum Cav.) is a subtropical solanaceous tree with increasing agronomic interest due to its nutritious edible fruits. Growing demand for tamarillo plants and fruits requires optimization of existing propagation methods and scaled-up systems for large-scale cloning of selected germplasm. Three in vitro protocols have been used to micropropagate tamarillo: (1) axillary shoot proliferation in a semisolid medium, (2) organogenesis, and (3) somatic embryogenesis procedures. Variables such as the age of the established shoot cultures and rooting treatments were also analyzed. The morphological and physiological quality of acclimatized plants derived from all the methodologies were compared, with seed-derived plants used as a control group. Overall, the results show that in vitro-derived plants have a similar development to seed-derived plants. Micropropagation by axillary shoot proliferation was highly efficient, with rooting rates above 80% in most treatments. Organogenesis induction was more effective from lamina explants using MS media with 2.0 mg·L−1 6-benzylaminopurine. Both organogenesis and somatic embryogenesis-derived plants were also morphologically and physiologically equivalent to seed and axillary shoot-derived plants. The specificities of each micropropagation method are discussed.
Funder
Foundation for Science and Technology European Regional Development Fund FCT/MCTES
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference53 articles.
1. Nutraceutical properties of tamarillo fruits: A vibrational study;Martin;Spectrochim. Acta Part A Mol. Biomol. Spectrosc.,2021 2. Rito, M., Marques, J., Costa, R., Correia, S., Lopes, T., Martin, D., Canhoto, J., Carvalho, L., and Marques, M.P. (2023). Antioxidant potential of tamarillo fruits—Chemical and infrared spectroscopy analysis. Antioxidants, 12. 3. (2022, December 23). Tridge. Available online: https://www.tridge.com/intelligences/tamarillo/export. 4. Correia, S., Braga, A., Martins, J., Correia, B., Pinto, G., and Canhoto, J. (2023). Effects of polyploidy on physiological performance of acclimatized Solanum betaceum Cav. plants under water deficit. Forests, 14. 5. Bajaj, Y.P.S. (1996). Biotechnology in Agriculture and Forestry, Trees IV, Springer.
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