BrCYP71A15 Negatively Regulates Hg Stress Tolerance by Modulating Cell Wall Biosynthesis in Yeast
Author:
Anwar Ali1ORCID, Zhang Shu1, Wang Lixia1, He Lilong1, Gao Jianwei1ORCID
Affiliation:
1. Institute of Vegetables, Shandong Academy of Agricultural Sciences, Jinan 250100, China
Abstract
Over the past two decades, heavy metal pollution has been a common problem worldwide, greatly threatening crop production. As one of the metal pollutants, Mercury (Hg) causes damage to plant cells and reduces cellular and biochemical activities. In this study, we identified a novel cytochrome P450 family gene, BrCYP71A15, which was involved in Hg stress response in yeast. In Chinese cabbage, the BrCYP71A15 gene was located on chromosome A01, which was highly expressed in roots. Additionally, the expression level of BrCYP71A15 was induced by different heavy metal stresses, and the BrCYP71A15 protein exhibited a strong interaction with other proteins. Overexpression of BrCYP71A15 in yeast cells showed no response to a number of heavy metal stresses (Cu, Al, Co, Cd) in yeast but showed high sensitivity to Hg stress; the cells grew slower than those carrying the empty vector (EV). Moreover, upon Hg stress, the growth of the BrCYP71A15-overexpressing cells increased over time, and Hg accumulation in yeast cells was enhanced by two-fold compared with the control. Additionally, BrCYP71A15 was translocated into the nucleus under Hg stress. The expression level of cell wall biosynthesis genes was significantly influenced by Hg stress in the BrCYP71A15-overexpressing cells. These findings suggested that BrCYP71A15 might participate in HG stress tolerance. Our results provide a fundamental basis for further genome editing research and a novel approach to decrease Hg accumulation in vegetable crops and reduce environmental risks to human health through the food chain.
Funder
Natural Science Foundation of Shandong Province Technology projects of the China Huaneng Group Co., Ltd. National Natural Science Foundation, China Projects of 20 Rules for New Universities in Jinan, China Modern Agricultural Industrial Technology System Funding of Shandong Province, China China Agriculture Research System Agricultural Science and Technology Innovation Project of SAAS
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference36 articles.
1. Accumulation capacity of ions in cabbage (Brassica oleracea L.) supplied with sea water;Gu;Plant Soil Environ.,2016 2. Quantification of glucosinolates, anthocyanins, free amino acids, and vitamin C in inbred lines of cabbage (Brassica oleracea L.);Park;Food Chem.,2014 3. Yang, L., Wu, Y., Wang, X., Lv, J., Tang, Z., Hu, L., Luo, S., Wang, R., Ali, B., and Yu, J. (2022). Physiological Mechanism of Exogenous 5-Aminolevulinic Acid Improved the Tolerance of Chinese Cabbage (Brassica pekinensis L.) to Cadmium Stress. Front. Plant Sci., 13. 4. Anwar, A., Zhang, S., Wang, L.X., Wang, F., He, L., and Gao, J. (2022). Genome-Wide Identification and Characterization of Chinese Cabbage S1fa Transcription Factors and Their Roles in Response to Salt Stress. Antioxidants, 11. 5. Heavy metal-regulated new microRNAs from rice;Huang;J. Inorg. Biochem.,2009
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