Effects of Polyethylene Microplastics and Phenanthrene on Soil Properties, Enzyme Activities and Bacterial Communities

Abstract

Microplastics (MPs) or polycyclic aromatic hydrocarbons (PAHs) pollution has received increasing concern due to their ubiquitous distribution and potential risks in soils. However, nothing is known about the influences of PAHs-MPs combined pollution on soil ecosystems. To address the knowledge gap, a 1-year soil microcosm experiment was conducted to systematically investigate the single and combined effect of polyethylene (PE) /phenanthrene (PHE) on soil chemical properties, enzymatic activities and bacterial communities (i.e., diversity, composition and function). Results showed that PE and PHE-PE significantly decreased soil pH. The available phosphorus (AP) and neutral phosphatase activity were not considerably changed by PHE, PE and PHE-PE. Significant enhancement of dehydrogenase activity in a PHE-PE amended system might be due to the degradation of PHE by indigenous bacteria (i.e., Sphingomonas, Sphingobium), and PE could enhance this stimulative effect. PHE and PHE-PE led to a slight increase in soil organic matter (SOM) and fluorescein diacetate hydrolase (FDAse) activity but a decrease in available nitrogen (AN) and urease activity. PE significantly enhanced the functions of nitrogen cycle and metabolism, reducing SOM/AN contents but increasing urease/FDAse activities. There were insignificant impacts on overall community diversity and composition in treated samples, although some bacterial genera were significantly stimulated or attenuated with treatments. In conclusion, the addition of PHE and PE influenced the soil chemical properties, enzymatic activities and bacterial community diversity/composition to some extent. The significantly positive effect of PE on the nitrogen cycle and on metabolic function might lead to the conspicuous alterations in SOM/AN contents and urease/FDAse activities. This study may provide new basic information for understanding the ecological risk of PAHs-MPs combined pollution in soils.