RNA-Seq and Comparative Transcriptomic Analyses of Asian Soybean Rust Resistant and Susceptible Soybean Genotypes Provide Insights into Identifying Disease Resistance Genes
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Published:2023-08-30
Issue:17
Volume:24
Page:13450
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Hao Qingnan12, Yang Hongli12, Chen Shuilian12, Qu Yanhui13ORCID, Zhang Chanjuan12, Chen Limiao12, Cao Dong12ORCID, Yuan Songli12ORCID, Guo Wei12, Yang Zhonglu12, Huang Yi12, Shan Zhihui12, Chen Haifeng12, Zhou Xinan12ORCID
Affiliation:
1. Institute of Oil Crops Research, Chinese Academy of Agriculture Sciences, Wuhan 430062, China 2. Key Laboratory for Biological Sciences of Oil Crops, Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China 3. The Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract
Asian soybean rust (ASR), caused by Phakopsora pachyrhizi, is one of the most destructive foliar diseases that affect soybeans. Developing resistant cultivars is the most cost-effective, environmentally friendly, and easy strategy for controlling the disease. However, the current understanding of the mechanisms underlying soybean resistance to P. pachyrhizi remains limited, which poses a significant challenge in devising effective control strategies. In this study, comparative transcriptomic profiling using one resistant genotype and one susceptible genotype was performed under infected and control conditions to understand the regulatory network operating between soybean and P. pachyrhizi. RNA-Seq analysis identified a total of 6540 differentially expressed genes (DEGs), which were shared by all four genotypes. The DEGs are involved in defense responses, stress responses, stimulus responses, flavonoid metabolism, and biosynthesis after infection with P. pachyrhizi. A total of 25,377 genes were divided into 33 modules using weighted gene co-expression network analysis (WGCNA). Two modules were significantly associated with pathogen defense. The DEGs were mainly enriched in RNA processing, plant-type hypersensitive response, negative regulation of cell growth, and a programmed cell death process. In conclusion, these results will provide an important resource for mining resistant genes to P. pachyrhizi infection and valuable resources to potentially pyramid quantitative resistance loci for improving soybean germplasm.
Funder
the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences the Basic research fund of the Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences the National Natural Science Foundation of China
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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