Cut, Root, and Grow: Simplifying Cassava Propagation to Scale

Author:

Sheat Samar1ORCID,Mushi Edda2,Gwandu Francisca3,Sikirou Mouritala4ORCID,Baleke Patrick5,Kayondo Siraj Ismail2ORCID,Kulembeka Heneriko3,Adetoro Najimu4ORCID,Winter Stephan1ORCID

Affiliation:

1. Plant Virus Department, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany

2. International Institute of Tropical Agriculture (IITA), Dar es Salaam P.O. Box 3444, Tanzania

3. Tanzania Agricultural Research Institute, TARI Ukiriguru, Mwanza P.O. Box 1433, Tanzania

4. International Institute of Tropical Agriculture (IITA), Kalambo Bukavu P.O. Box 4163, Democratic Republic of the Congo

5. Association Volontaire pour le Développement (AVPD), Karenzu, Luvungi, Itara 1, Democratic Republic of the Congo

Abstract

Cassava (Manihot esculenta Crantz) is an essential crop with increasing importance for food supply and as raw material for industrial processing. The crop is vegetatively propagated through stem cuttings taken at the end of the growing cycle and its low multiplication rate and the high cost of stem transportation are detrimental to the increasing demand for high-quality cassava planting materials. Rapid multiplication of vegetative propagules of crops comprises tissue culture (TC) and semi-autotroph hydroponics (SAH) that provide cost-effective propagation of plant materials; however, they contrast the need for specific infrastructure, special media and substrates, and trained personnel. Traditional methods such as TC and SAH have shown promise in efficient plant material propagation. Nonetheless, these techniques necessitate specific infrastructure, specialized media and substrates, as well as trained personnel. Moreover, losses during the intermediate nursery and adaptation stages limit the overall effectiveness of these methods. Building upon an earlier report from Embrapa Brazil, which utilized mature buds from cassava for rapid propagation, we present a modified protocol that simplifies the process for wider adoption. Our method involves excising single nodes with attached leaves from immature (green) cassava stems at 2 months after planting (MAP). These nodes are then germinated in pure water, eliminating the need for specific growth substrates and additional treatments. After the initial phase, the rooted sprouts are transferred into soil within 1–8 weeks. The protocol demonstrates a high turnover rate at minimal costs. Due to its simplicity, cost-effectiveness, and robustness, this method holds significant promise as an efficient means of producing cassava planting materials to meet diverse agricultural needs.

Funder

Bill & Melinda Gates Foundation

Publisher

MDPI AG

Subject

Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics

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