Integrated Workflow for the Label-Free Isolation and Genomic Analysis of Single Circulating Tumor Cells in Pancreatic Cancer
-
Published:2022-07-16
Issue:14
Volume:23
Page:7852
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Rupp Brittany,
Owen SarahORCID,
Ball HarrisonORCID,
Smith Kaylee JudithORCID,
Gunchick Valerie,
Keller Evan T.ORCID,
Sahai VaibhavORCID,
Nagrath SunithaORCID
Abstract
As pancreatic cancer is the third deadliest cancer in the U.S., the ability to study genetic alterations is necessary to provide further insight into potentially targetable regions for cancer treatment. Circulating tumor cells (CTCs) represent an especially aggressive subset of cancer cells, capable of causing metastasis and progressing the disease. Here, we present the Labyrinth–DEPArray pipeline for the isolation and analysis of single CTCs. Established cell lines, patient-derived CTC cell lines and freshly isolated CTCs were recovered and sequenced to reveal single-cell copy number variations (CNVs). The resulting CNV profiles of established cell lines showed concordance with previously reported data and highlight several gains and losses of cancer-related genes such as FGFR3 and GNAS. The novel sequencing of patient-derived CTC cell lines showed gains in chromosome 8q, 10q and 17q across both CTC cell lines. The pipeline was used to process and isolate single cells from a metastatic pancreatic cancer patient revealing a gain of chromosome 1q and a loss of chromosome 5q. Overall, the Labyrinth-DEPArray pipeline offers a validated workflow combining the benefits of antigen-free CTC isolation with single cell genomic analysis.
Funder
National Institutes of Health
National Science Foundation
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference58 articles.
1. Cancer Stat Facts: Pancreatic Cancer. National Cancer Institute Surveillance, Epidemiology and End Results Program,2022
2. Cancer Stat Facts: Common Cancer Sites. National Cancer Institute Surveillance, Epidemiology and End Results Program,2022
3. How is pancreatic cancer diagnosed? Pancreatic Cancer UK
https://www.pancreaticcancer.org.uk/information/how-is-pancreatic-cancer-diagnosed/#:~:text=Pancreatic%20cancer%20can%20be%20difficult,symptoms%20can%20be%20quite%20vague
4. Epidemiology of Pancreatic Cancer: Global Trends, Etiology and Risk Factors
5. The Importance of Biopsy in the Era of Molecular Medicine
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献