Biochemical Properties of Black and Green Teas and Their Insoluble Residues as Natural Dietary Additives to Optimize In Vitro Rumen Degradability and Fermentation but Reduce Methane in Sheep

Abstract

Black (BTL) or green (GTL) tea and their spent tea (STL) leaves can be used as natural dietary additives for ruminants. Experiment 1 used a 3 × 2 × 2 factorial arrangement, with four replicates (n = 4) to test the effects of three different inclusions of tea leaves at 0 (control), 50, and 100 g/kg DM of two different tea types (BTL and GTL) in two different total mixed diets containing either ryegrass hay (RH) or rice straw (RS) on in vitro rumen organic matter degradability (IVOMD), volatile fatty acids (VFA), pH, ammonia (NH3), and methane (CH4) outputs over a 24 h incubation time. Experiment 2 followed a 3 × 2 × 2 factorial arrangement, with eight replicates (n = 8) to study the impacts of three different STL inclusions at 0, 100, and 200 g/kg DM of two different STL types (black and green) into two different total mixed diets containing either RH or RS on the same in vitro measurements. Both types of tea leaves decreased NH3 (p < 0.001) and CH4 (p < 0.01) without affecting (p > 0.05) rumen degradability, but the effect of their STL was less remarkable. Tea leaves and their STL inclusions improved (p < 0.01 and p < 0.001, respectively) the acetate to propionate (A:P) ratio. Compared with BTL, GTL containing diets had higher IVOMD (p < 0.05) and A:P ratio (p < 0.05) but lower NH3 (p < 0.001). Reduced rumen NH3 and CH4 outputs can be useful for protein and energy use efficiency while an increased A:P ratio might lead to increased milk fat synthesis and reduced low-fat milk syndrome. The surplus or wasted tea leaf products could be used as sustainable sources of nutrients to optimize rumen function and minimize environmental impacts of feeding ruminant animals.