Ovicidal activity and cytotoxicity of ethanolic extract of turmeric (Curcuma longa) and green tea (Camellia sinensis) to treat digestive parasite of sheep

Abstract

Background: The Trichuris eggs are collected from naturally infected sheep. Natural antihelmintics such as herbal medicines are needed as an alternative, such as natural compounds from endemic plants. Aim: This present study aims to evaluate the ovicidal activity and cytotoxicity effects of ethanolic extract of Curcuma longa (EECL) and Camelia sinensis (EECS) as a biological anthelmintic against the egg of Trichuris sp. Methods: The Trichuris eggs are collected from naturally infected sheep. CMC-Na solution 1% was used as control. The treatments were 0.12% EECL; 0.24% EECL; 0.15% EECS; 0.30% EECS; combination of 0.12% EECL and 0.30% EECS; combination 0.24% EECL; and 0.15% EECS. Ovicidal activity testing by microscopic examination of eggs treated using different concentrations of EECL extract, EECS and a combination of them. They were exposed for various times (7, 14, 21 and 28 days) and incubated at room temperature. Results: The study shows that combination of Curcuma longa extract and tea extract exhibits good ovicidal anthelmintic activity against the Trichuris sp. in sheep. Cytotoxicity examination using the 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide (MTT) test. Based on MTT data processed using regression analysis, the obtained LC50 from administration of EECL, EECS and a combination of both in a ratio of 1:1, 2:2, 1:2 and 2:1. The combination of EECL extract and EECS with the highest concentration produced cell viability of 28.46%, 17.25%, 56.01%, and 46.47%, respectively. Conclusion: It can be concluded that the most cytotoxic ingredient is found in the combination of EECL and EECS (2:2) at 17.25% and the safest is in the ratio (1:2) at 56.01%.