Differential CFTR-Interactome Proximity Labeling Procedures Identify Enrichment in Multiple SLC Transporters

Author:

Chevalier Benoît,Baatallah Nesrine,Najm MatthieuORCID,Castanier Solène,Jung VincentORCID,Pranke Iwona,Golec AnitaORCID,Stoven VéroniqueORCID,Marullo StefanoORCID,Antigny FabriceORCID,Guerrera Ida ChiaraORCID,Sermet-Gaudelus Isabelle,Edelman Aleksander,Hinzpeter Alexandre

Abstract

Proteins interacting with CFTR and its mutants have been intensively studied using different experimental approaches. These studies provided information on the cellular processes leading to proper protein folding, routing to the plasma membrane, recycling, activation and degradation. Recently, new approaches have been developed based on the proximity labeling of protein partners or proteins in close vicinity and their subsequent identification by mass spectrometry. In this study, we evaluated TurboID- and APEX2-based proximity labeling of WT CFTR and compared the obtained data to those reported in databases. The CFTR-WT interactome was then compared to that of two CFTR (G551D and W1282X) mutants and the structurally unrelated potassium channel KCNK3. The two proximity labeling approaches identified both known and additional CFTR protein partners, including multiple SLC transporters. Proximity labeling approaches provided a more comprehensive picture of the CFTR interactome and improved our knowledge of the CFTR environment.

Funder

Agence Nationale de la Recherche

Vaincre la Mucoviscidose

Fondation Dassault Systems

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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