Transcriptomic and Proteomic Analyses Unveil the Role of Nitrogen Metabolism in the Formation of Chinese Cabbage Petiole Spot
-
Published:2024-01-23
Issue:3
Volume:25
Page:1366
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Mei Ying1, Lei Juanli1, Liu Wenqi12, Yue Zhichen1, Hu Qizan1, Tao Peng1, Li Biyuan1, Zhao Yanting1
Affiliation:
1. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China 2. College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, China
Abstract
Chinese cabbage is the most widely consumed vegetable crop due to its high nutritional value and rock-bottom price. Notably, the presence of the physiological disease petiole spot significantly impacts the appearance quality and marketability of Chinese cabbage. It is well known that excessive nitrogen fertilizer is a crucial factor in the occurrence of petiole spots; however, the mechanism by which excessive nitrogen triggers the formation of petiole spots is not yet clear. In this study, we found that petiole spots initially gather in the intercellular or extracellular regions, then gradually extend into intracellular regions, and finally affect adjacent cells, accompanied by cell death. Transcriptomic and proteomic as well as physiology analyses revealed that the genes/proteins involved in nitrogen metabolism exhibited different expression patterns in resistant and susceptible Chinese cabbage lines. The resistant Chinese cabbage line has high assimilation ability of NH4+, whereas the susceptible one accumulates excessive NH4+, thus inducing a burst of reactive oxygen species (ROS). These results introduce a novel perspective to the investigation of petiole spot induced by the nitrogen metabolism pathway, offering a theoretical foundation for the development of resistant strains in the control of petiole spot.
Funder
Zhejiang Province Research and Development Program of. “Lingyan” Zhejiang province Major science and technology Program Zhejiang Provincial Natural Science Foundation
Reference41 articles.
1. Glucosinolate metabolism, functionality and breeding for the improvement of Brassicaceae vegetables;Ishida;Breed. Sci.,2014 2. Cao, W., Wang, P., Yang, L., Fang, Z., Zhang, Y., Zhuang, M., Lv, H., Wang, Y., and Ji, J. (2021). Carotenoid biosynthetic genes in cabbage: Genome-wide identification, evolution, and expression analysis. Genes, 12. 3. Identification of Key Gene Networks Controlling Soluble Sugar and Organic Acid Metabolism During Oriental Melon Fruit Development by Integrated Analysis of Metabolic and Transcriptomic Analyses;Cheng;Front. Plant Sci.,2022 4. Lee, D.H., Lim, J.-A., Lee, J., Roh, E., Jung, K., Choi, M., Oh, C., Ryu, S., Yun, J., and Heu, S. (2013). Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage. Microbiology, 159. 5. Li, L., Guo, B., Feng, C., Liu, H., and Lin, D. (2022). Growth, physiological, and temperature characteristics in chinese cabbage pakchoi as affected by Cd-stressed conditions and identifying its main controlling factors using PLS model. BMC Plant Biol., 22.
|
|