Affiliation:
1. Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, Republic of Korea
2. College of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, Republic of Korea
Abstract
Obesity is acknowledged as a significant risk factor for cardiovascular disease, often accompanied by increased inflammation and diabetes. Bioactive peptides derived from marine animal proteins show promise as safe and effective anti-obesity agents by regulating adipocyte differentiation through the AMPK signaling pathway. Therefore, this study aims to investigate the anti-obesity and anti-diabetic effects of bioactive compounds derived from a Meretrix lusoria Protamex enzymatic hydrolysate (MLP) fraction (≤1 kDa) through a 6-week treatment (150 mg/kg or 300 mg/kg, administered once daily) in leptin receptor-deficient db/db mice. The MLP treatment significantly decreased the body weight, serum total cholesterol, triglycerides, and LDL-cholesterol levels while also exhibiting a beneficial effect on hepatic and serum marker parameters in db/db mice. A histological analysis revealed a reduction in hepatic steatosis and epididymal fat following MLP treatment. Furthermore, poor glucose tolerance was improved, and hepatic antioxidant enzyme activities were elevated in MLP-treated mice compared to db/db control mice. Western blot analysis showed an increased expression of the AMPK protein after MLP treatment. In addition, the expression of lipogenic genes decreased in db/db mice. These findings indicate that bioactive peptides, which are known to regulate blood glucose levels, lipid metabolism, and adipogenesis, could be beneficial functional food additives and pharmaceuticals.
Funder
National Research Foundation of Korea
Reference63 articles.
1. Bicyclol alleviates obesity-induced renal injury by inhibiting JNK and NF-κB-mediated inflammation;Zhang;Int. Immunopharmacol.,2024
2. Signaling pathways in obesity: Mechanisms and therapeutic interventions;Wen;Signal Transduct. Target. Ther.,2022
3. Humans against obesity: Who will win?;Caballero;Adv. Nutr.,2019
4. Green, M., Arora, K., and Prakash, S. (2020). Microbial medicine: Prebiotic and probiotic functional foods to target obesity and metabolic syndrome. Int. J. Mol. Sci., 21.
5. db/db mice exhibit features of human type 2 diabetes that are not present in weight-matched C57BL/6J mice fed a western diet;Burke;J. Diabetes Res.,2017