The Antioxidant Properties of Glucosinolates in Cardiac Cells Are Independent of H2S Signaling
-
Published:2024-01-05
Issue:2
Volume:25
Page:696
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Harvey Félix12, Aromokunola Boluwaji12ORCID, Montaut Sabine1ORCID, Yang Guangdong12ORCID
Affiliation:
1. School of Natural Sciences, Laurentian University, Sudbury, ON P3E 2C6, Canada 2. Cardiovascular and Metabolic Research Unit, Laurentian University, Sudbury, ON P3E 2C6, Canada
Abstract
The organic sulfur-containing compounds glucosinolates (GSLs) and the novel gasotransmitter H2S are known to have cardioprotective effects. This study investigated the antioxidant effects and H2S-releasing potential of three GSLs ((3E)-4-(methylsulfanyl)but-3-enyl GSL or glucoraphasatin, 4-hydroxybenzyl GSL or glucosinalbin, and (RS)-6-(methylsulfinyl)hexyl GSL or glucohesperin) in rat cardiac cells. It was found that all three GSLs had no effect on cardiac cell viability but were able to protect against H2O2-induced oxidative stress and cell death. NaHS, a H2S donor, also protected the cells from H2O2-stimulated oxidative stress and cell death. The GSLs alone or mixed with cysteine, N-acetylcysteine, glutathione, H2O2, iron and pyridoxal-5′-phosphate, or mouse liver lysates did not induce H2S release. The addition of GSLs also did not alter endogenous H2S levels in cardiac cells. H2O2 significantly induced cysteine oxidation in the cystathionine gamma-lyase (CSE) protein and inhibited the H2S production rate. In conclusion, this study found that the three tested GSLs protect cardiomyocytes from oxidative stress and cell death but independently of H2S signaling.
Funder
Natural Sciences and Engineering Research Council Heart and Stroke Foundation of Canada
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference38 articles.
1. Miękus, N., Marszałek, K., Podlacha, M., Iqbal, A., Puchalski, C., and Świergiel, A.H. (2020). Health benefits of plant-derived sulfur compounds, glucosinolates, and organosulfur compounds. Molecules, 25. 2. Kamal, R.M., Abdull Razis, A.F., Mohd Sukri, N.S., Perimal, E.K., Ahmad, H., Rollin, P., Djedaini-Pilard, F., Mazzon, E., and Rigaud, S. (2022). Beneficial health effects of glucosinolates-derived isothiocyanates on cardiovascular and neurodegenerative diseases. Molecules, 27. 3. Glucosinolate diversity analysis in Choy Sum (Brassica rapa subsp. chinensis var. parachinensis) Germplasms for functional food breeding;Kim;Foods,2023 4. Glucosinolates and isothiocyanates in health and disease;Kostov;Trends Mol. Med.,2012 5. Diet rich in high glucoraphanin broccoli reduces plasma LDL cholesterol: Evidence from randomised controlled trials;Armah;Mol. Nutr. Food Res.,2015
|
|