Affiliation:
1. Guangdong Provincial Key Laboratory of Crop Genetic Improvement, South China Peanut Sub-Center of National Center of Oilseed Crops Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences (GDAAS), Guangzhou 510640, China
2. College of Agriculture, South China Agriculture University, Guangzhou 510642, China
3. WA State Agricultural Biotechnology Centre, Centre for Crop and Food Innovation, Food Futures Institute, Murdoch University (MU), Murdoch, WA 6150, Australia
4. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China
Abstract
Fatty Acid Desaturase 2 (FAD2) controls the conversion of oleic acids into linoleic acids. Mutations in FAD2 not only increase the high-oleic content, but also repress the leaf growth. However, the mechanism by which FAD2 regulates the growth pathway has not been elucidated in peanut leaves with single-cell resolution. In this study, we isolated fad2 mutant leaf protoplast cells to perform single-cell RNA sequencing. Approximately 24,988 individual cells with 10,249 expressed genes were classified into five major cell types. A comparative analysis of 3495 differentially expressed genes (DEGs) in distinct cell types demonstrated that fad2 inhibited the expression of the cytokinin synthesis gene LOG in vascular cells, thereby repressing leaf growth. Further, pseudo-time trajectory analysis indicated that fad2 repressed leaf cell differentiation, and cell-cycle evidence displayed that fad2 perturbed the normal cell cycle to induce the majority of cells to drop into the S phase. Additionally, important transcription factors were filtered from the DEG profiles that connected the network involved in high-oleic acid accumulation (WRKY6), activated the hormone pathway (WRKY23, ERF109), and potentially regulated leaf growth (ERF6, MYB102, WRKY30). Collectively, our study describes different gene atlases in high-oleic and normal peanut seedling leaves, providing novel biological insights to elucidate the molecular mechanism of the high-oleic peanut-associated agronomic trait at the single-cell level.
Funder
The Open Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan in Guangdong Province
Guangdong Provincial Key Research and Development Program-Modern Seed Industry
Guangzhou Basic and Applied Basic Research Foundation
The National Natural Science Foundation of China
China Agriculture Research System of MOF and MARA
Guangdong Basic and Applied Basic Research Foundation
Technology Special Fund of Guangdong Province Agriculture and Rural Affairs Department
Special Project for Rural Revitalization Strategy in Guangdong Province
Agricultural Competitive Industry Discipline Team Building Project of Guangdong Academy of Agricultural Sciences
Special Fund for Scientific Innovation Strategy-Construction of High Level Academy of Agriculture Science
The Foundation of Director of Crop Research Institute of Guangdong Academy of Agriculture Sciences
Open Fund of Guangdong Provincial Key Laboratory of Crop Genetic Improvement
The Science and Technology Planning Project of Guangdong Province
The Special Support Program of Guangdong Province
Rural Revitalization Strategy Special Foundation GDAAS Specialist Working Station
Planting Industry Development Program of 2023 rural revitalization strategy Special Project
Science and Technology Planning Project of Heyuan City
Science and Technology Planning Project of Qingyuan City
Food Futures Institute, Murdoch University