Prospective Application of Nanoencapsulated Bacillus amyloliquefaciens on Broiler Chickens’ Performance and Gut Health with Efficacy against Campylobacter jejuni Colonization
Author:
Ismail Hesham12ORCID, Ibrahim Doaa3ORCID, El Sayed Shorouk4, Wahdan Ali5, El-Tarabili Reham M.5ORCID, Rizk El-Ghareeb Waleed16ORCID, Abdullah Alhawas Bassam1ORCID, Alahmad Badr Abdul-Hakim Y.1, Abdel-Raheem Sherief M.17, El-Hamid Marwa I. Abd4ORCID
Affiliation:
1. Department of Public Health, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Hofuf 31982, Al-Ahsa, Saudi Arabia 2. Food Hygiene Department, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt 3. Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt 4. Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt 5. Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt 6. Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt 7. Department of Animal Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt
Abstract
Probiotics as novel antibiotics’ substitutes are verified to provide barriers for hindering the colonization of enteric bacterial pathogens with nutritional benefits. For enhancement of the probiotics’ effectiveness, their integration within nanomaterials is a paramount tool to support the progress of new compounds with functional features. Therefore, we addressed the impact of effective delivery of probiotics (Bacillus amyloliquefaciens) loaded nanoparticles (BNPs) on performance and Campylobacter jejuni (C. jejuni) shedding and colonization in poultry. Two hundred Ross broiler chickens were divided into four groups fed various BNP levels: BNPs I, BNPs II, BNPs III, and BNPs-free diets for 35 days. Nanoparticles delivery of probiotics within broiler diets improved growth performance as reflected by higher body weight gain and superior feed conversion ratio, especially in BNPs II- and BNPs III-fed groups. In parallel, the mRNA expression levels of digestive enzymes encoding genes (AMY2a, PNLIP, CELA1, and CCK) achieved their peaks in BNPs III-fed group (1.69, 1.49, 1.33, and 1.29-fold change, respectively) versus the control one. Notably, with increasing the levels of BNPs, the abundance of beneficial microbiota, such as Bifidobacterium and Lactobacillus species, was favored over harmful ones, including Clostridium species and Enterobacteriaceae. Birds fed higher levels of BNPs displayed significant improvement in the expression of barrier functions-linked genes including DEFB1, FABP-2, and MUC-2 alongside substantial reduction in cecal colonization and fecal shedding of C. jejuni. From the aforementioned positive effects of BNPs, we concluded their potential roles as growth promoters and effective preventive aids for C. jejuni infection in poultry.
Funder
Deputyship for Research and Innovation, Ministry of Education in Saudi Arabia
Subject
General Veterinary,Animal Science and Zoology
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