Amitraz Resistance in French Varroa Mite Populations—More Complex Than a Single-Nucleotide Polymorphism
Author:
Marsky Ulrike1, Rognon Bénédicte2, Douablin Alexandre2, Viry Alain3, Rodríguez Ramos Miguel Angel1, Hammaidi Abderrahim1
Affiliation:
1. Véto-Pharma, 12 Rue de la Croix Martre, 91120 Palaiseau, France 2. Biomnigene, 18 Rue Alain Savary, 25000 Besançon, France 3. LDA39, Laboratoire Départemental d’Analyses du Jura, 59 Rue du Vieil Hôpital, 39800 Poligny, France
Abstract
Resistance against amitraz in Varroa mite populations has become a subject of interest in recent years due to the increasing reports of the reduced field efficacy of amitraz treatments, especially from some beekeepers in France and the United States. The loss of amitraz as a reliable tool to effectively reduce Varroa mite infestation in the field could severely worsen the position of beekeepers in the fight to keep Varroa infestation rates in their colonies at low levels. In this publication, we present data from French apiaries, collected in the years 2020 and 2021. These data include the field efficacy of an authorized amitraz-based Varroa treatment (Apivar® ,Véto-pharma, France) and the results of laboratory sensitivity assays of Varroa mites exposed to the reference LC90 concentration of amitraz. In addition, a total of 240 Varroa mites from Eastern, Central, and Southern regions in France that were previously classified as either “sensitive” or “resistant” to amitraz in a laboratory sensitivity assay were genotyped. The genetic analyses of mite samples are focused on the β-adrenergic-like octopamine receptor, which is considered as the main target site for amitraz in Varroa mites. Special attention was paid to a single-nucleotide polymorphism (SNP) at position 260 of the ORβ-2R-L gene that was previously associated to amitraz resistance in French Varroa mites, Varroa. Our findings confirm that amitraz resistance occurs in patches or “islands of resistance”, with a less severe reduction in treatment efficacy compared to pyrethroid resistance or coumaphos resistance in Varroa mites. The results of our genetic analyses of Varroa mites call into question the hypothesis of the SNP at position 260 of the ORβ-2R-L gene being directly responsible for amitraz resistance development.
Reference59 articles.
1. Mondet, F., Parejo, M., Meixner, M.D., Costa, C., Kryger, P., Andonov, S., Servin, B., Basso, B., Bieńkowska, M., and Bigio, G. (2020). Evaluation of suppressed mite reproduction (SMR) reveals potential for Varroa resistance in European honey bees (Apis mellifera L.). Insects, 11. 2. A national survey of managed honey bee colony losses in the USA: Results from the Bee Informed Partnership for 2017–2018, 2018–2019, and 2019–2020;Bruckner;J. Apic. Res.,2023 3. Stahlmann-Brown, P., Hall, R.J., Pragert, H., and Robertson, T. (2022). Varroa appears to drive persistent increases in New Zealand colony losses. Insects, 13. 4. Mutinelli, F., Pinto, A., Barzon, L., and Toson, M. (2022). Some considerations about winter colony losses in Italy according to the Coloss questionnaire. Insects, 13. 5. Land use changes associated with declining honey bee health across temperate North America;Rodney;Environ. Res. Lett.,2023
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