Author:
McMenamin Alexander J.,Parekh Fenali,Lawrence Verena,Flenniken Michelle L.
Abstract
Honey bee (Apis mellifera) health is impacted by viral infections at the colony, individual bee, and cellular levels. To investigate honey bee antiviral defense mechanisms at the cellular level we further developed the use of cultured primary cells, derived from either larvae or pupae, and demonstrated that these cells could be infected with a panel of viruses, including common honey bee infecting viruses (i.e., sacbrood virus (SBV) and deformed wing virus (DWV)) and an insect model virus, Flock House virus (FHV). Virus abundances were quantified over the course of infection. The production of infectious virions in cultured honey bee pupal cells was demonstrated by determining that naïve cells became infected after the transfer of deformed wing virus or Flock House virus from infected cell cultures. Initial characterization of the honey bee antiviral immune responses at the cellular level indicated that there were virus-specific responses, which included increased expression of bee antiviral protein-1 (GenBank: MF116383) in SBV-infected pupal cells and increased expression of argonaute-2 and dicer-like in FHV-infected hemocytes and pupal cells. Additional studies are required to further elucidate virus-specific honey bee antiviral defense mechanisms. The continued use of cultured primary honey bee cells for studies that involve multiple viruses will address this knowledge gap.
Funder
Project Apis m.
National Science Foundation
Reference193 articles.
1. Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell Culture;van Cleef,2011
2. Drosophila Cell Lines as Model Systems and as an Experimental Tool;Baum,2008
3. Human stem cell models to study host–virus interactions in the central nervous system
4. Infectious Sindbis virus transient expression vectors for studying antigen processing and presentation.
5. Vaccine Production;Genzel,2007
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