The Additional 15 nt of 5′ UTR in a Novel Recombinant Isolate of Chilli Veinal Mottle Virus in Solanum nigrum L. Is Crucial for Infection-Reference-Cited by-同舟云学术

The Additional 15 nt of 5′ UTR in a Novel Recombinant Isolate of Chilli Veinal Mottle Virus in Solanum nigrum L. Is Crucial for Infection

Published:2023-06-23 Issue:7 Volume:15 Page:1428
ISSN:1999-4915
Container-title:Viruses
language:en
Short-container-title:Viruses

Author:

Wan Qionglian¹²³⁴,Zheng Kaiyue²³,Wu Jian²³,Bu Shan²³,Jiao Mengting²³,Zhou Huijie²³,Lu Yuwen²³^ORCID,Zheng Hongying²³,Wu Guanwei²³^ORCID,Rao Shaofei²³,Chen Hairu¹,Yan Fei²³,Peng Jiejun²³^ORCID

Affiliation:

1. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China

2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Institute of Plant Virology, Ningbo University, Ningbo 315211, China

3. Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China

4. School of Chemistry, Biology and Environment, Yuxi Normal University, Yuxi 653100, China

Abstract

An isolate of chilli veinal mottle virus (ChiVMV; genus Potyvirus) of Solanum nigrum L. from southwest China (ChiVMV-YunN/Yuxi) was identified and sequenced (GenBank: OP404087). Comparison with other ChiVMV isolates and recombination analyses suggested a recombinant origin. The most significant recombination event among all 21 complete ChiVMV isolates was an ending breakpoint at 1408–1488 for ChiVMV-YunN/Yuxi with ChiVMV-TaiW and ChiVMV-YunN/Ca operating as the respective major and minor parents. Interestingly, the 5′ UTR of ChiVMV-YunN/Yuxi is 15 nucleotides (‘AAAAATAAAACAACC’) longer than other reported isolates. A full-length clone of ChiVMV-YunN/Yuxi was constructed and was shown to be infectious in Nicotiana benthamiana. The additional 15 nt of 5′ UTR in ChiVMV-YunN/Yuxi was stable when transmitted through three generations. Experiments with modified clones showed that the additional 15 nt are essential for infection by this isolate.

Funder

National Key R&D Program of China

Chinese Agriculture Research System of MOF and MARA

K. C. Wong education foundation

Publisher

MDPI AG

Subject

Virology,Infectious Diseases

Link

https://www.mdpi.com/1999-4915/15/7/1428/pdf

Reference29 articles.

1. The effect of Chilli veinal mottle virus on yield of Chilli (Capsicum annuum L.);Ong;MARDI Res. Bull.,1980

2. Characterization of a Pepper Vein Banding Virus from Chili Pepper in India;Ravi;Plant Dis.,1997

3. First Report of Chilli veinal mottle virus Infecting Tomato (Solanum lycopersicum) in China;Zhao;Plant Dis.,2014

4. Complete genomic RNA sequence of pepper-infecting Chilli veinal mottle virus Korean isolate;Ha;J. Plant Dis. Prot.,2013

5. Inter- and intralineage recombinants are common in natural populations of Turnip mosaic virus;Tan;J. Gen. Virol.,2004