Microgravity as an Anti-Metastatic Agent in an In Vitro Glioma Model
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Published:2023-11-25
Issue:4
Volume:3
Page:636-650
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ISSN:2673-4125
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Container-title:Biophysica
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language:en
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Short-container-title:Biophysica
Author:
Sabbatini Maurizio1ORCID, Bonetto Valentina1, Magnelli Valeria1ORCID, Lorusso Candida1ORCID, Dondero Francesco1ORCID, Masini Maria Angela1
Affiliation:
1. Department of Science and Innovation Technology (DISIT), Università del Piemonte Orientale—Via T. Michel 11, 15121 Alessandria, Italy
Abstract
Gravity is a primary physical force that has a profound influence on the stability of the cell cytoskeleton. In our research, we investigated the influence of microgravity on altering the cytoskeletal pathways of glioblastoma cells. The highly infiltrative behavior of glioblastoma is supported by cytoskeletal dynamics and surface proteins that allow glioblastoma cells to avoid stable connections with the tissue environment and other cells. Glioblastoma cell line C6 was exposed to a microgravity environment for 24, 48, and 72 h by 3D-RPM, a laboratory instrument recognized to reproduce the effect of microgravity in cell cultures. The immunofluorescence for GFAP, vinculin, and Connexin-43 was investigated as signals related to cytoskeleton dynamics. The polymerization of GFAP and the expression of focal contact structured by vinculin were found to be altered, especially after 48 and 72 h of microgravity. Connexin-43, involved in several intracellular pathways that critically promote cell motility and invasion of glioma cells, was found to be largely reduced following microgravity exposure. In conclusion, microgravity, by reducing the expression of Connexin-43, alters the architecture of specific cytoskeletal elements such as GFAP and increases the focal contact, which can induce a reduction in glioma cell mobility, thereby inhibiting their aggressive metastatic behavior.
Funder
UPO FAR2017_Sabbatini UPO FAR2017_Masini THOR—Associazione per il Sostegno alla Ricerca ONLUS
Subject
Materials Chemistry,Economics and Econometrics,Media Technology,Forestry
Reference62 articles.
1. Spaceflight affects neuronal morphology and alters transcellular degradation of neuronal debris in adult Caenorhabditis elegans;Laranjeiro;iScience,2021 2. Effect of microgravity on proliferation and differentiation of embryonic stem cells in an automated culturing system during the TZ-1 space mission;Lei;Cell Prolif.,2018 3. Prasad, B., Grimm, D., Strauch, S.M., Erzinger, G.S., Corydon, T.J., Lebert, M., Magnusson, N.E., Infanger, M., Richter, P., and Krüger, M. (2020). Influence of Microgravity on Apoptosis in Cells, Tissues, and Other Systems In Vivo and In Vitro. Int. J. Mol. Sci., 21. 4. Lin, X., Zhang, K., Wei, D., Tian, Y., Gao, Y., Chen, Z., and Qian, A. (2020). The Impact of Spaceflight and Simulated Microgravity on Cell Adhesion. Int. J. Mol. Sci., 21. 5. Clinorotation-induced weightlessness influences the cytoskeleton of glial cells in culture;Uva;Brain Res.,2002
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