Abstract
The liver is considered the major target organ affected by oral exposure to titanium dioxide nanoparticles (TiO2 NPs), but the mechanism of hepatotoxicity is not fully understood. This study investigated the effect of TiO2 NPs on the expression profile of long non-coding RNA (lncRNA) in hepatocytes and tried to understand the potential mechanism of hepatotoxicity through bioinformatics analysis. The human hepatocellular carcinoma cells (HepG2) were treated with TiO2 NPs at doses of 0–200 μg/mL for 48 h and then RNA sequencing was implemented. The differential lncRNAs between the control and TiO2 NPs-treated groups were screened, then the lncRNA–mRNA network and enrichment pathways were analyzed via multivariate statistics. As a result, 46,759 lncRNAs were identified and 129 differential lncRNAs were screened out. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the targeted mRNAs of those differential lncRNAs were enriched in the Hedgehog signaling pathway, Vasopressin-regulated water reabsorption, and Glutamatergic synapse. Moreover, two lncRNA–mRNA networks, including lncRNA NONHSAT256380.1-JRK and lncRNA NONHSAT173563.1-SMIM22, were verified by mRNA detection. This study demonstrated that an alteration in the lncRNA expression profile could be induced by TiO2 NPs and epigenetics may play an important role in the mechanism of hepatotoxicity.
Funder
National Natural Science Foundation of China
National Key R&D Program of the Ministry of Science and Technology of China
Subject
Chemical Health and Safety,Health, Toxicology and Mutagenesis,Toxicology
Reference66 articles.
1. Directly nanopatternable nanoporous titania—Application to cell growth engineering;Garoli;Microelectron. Eng.,2016
2. Applications and implications of nanotechnologies for the food sector;Chaudhry;Food Addit. Contam. Part A,2008
3. Mustansar Hussain, C. (2018). Handbook of Nanomaterials for Industrial Applications, Elsevier.
4. Antibacterial properties and toxicity from metallic nanomaterials;Vimbela;Int. J. Nanomed.,2017
5. Hamdy, N.M., Boseila, A.A., Ramadan, A., and Basalious, E.B. (2022). Iron Oxide Nanoparticles-Plant Insignia Synthesis with Favorable Biomedical Activities and Less Toxicity, in the “Era of the-Green”: A Systematic Review. Pharmaceutics, 14.
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