FTIR Analysis of Renal Tissue for the Assessment of Hypertensive Organ Damage and proANP31–67 Treatment

Author:

Pioppi Leonardo1ORCID,Tombolesi Niki1ORCID,Parvan Reza2ORCID,da Silva Gustavo Jose Justo2ORCID,Altara Raffaele2,Paolantoni Marco1,Morresi Assunta1,Sassi Paola1ORCID,Cataliotti Alessandro2

Affiliation:

1. Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy

2. Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, 0450 Oslo, Norway

Abstract

The kidneys are one of the main end organs targeted by hypertensive disease. Although the central role of the kidneys in the regulation of high blood pressure has been long recognized, the detailed mechanisms behind the pathophysiology of renal damage in hypertension remain a matter of investigation. Early renal biochemical alterations due to salt-induced hypertension in Dahl/salt-sensitive rats were monitored by Fourier-Transform Infrared (FTIR) micro-imaging. Furthermore, FTIR was used to investigate the effects of proANP31–67, a linear fragment of pro-atrial natriuretic peptide, on the renal tissue of hypertensive rats. Different hypertension-induced alterations were detected in the renal parenchyma and blood vessels by the combination of FTIR imaging and principal component analysis on specific spectral regions. Changes in amino acids and protein contents observed in renal blood vessels were independent of altered lipid, carbohydrate, and glycoprotein contents in the renal parenchyma. FTIR micro-imaging was found to be a reliable tool for monitoring the remarkable heterogeneity of kidney tissue and its hypertension-induced alterations. In addition, FTIR detected a significant reduction in these hypertension-induced alterations in the kidneys of proANP31–67-treated rats, further indicating the high sensitivity of this cutting-edge imaging modality and the beneficial effects of this novel medication on the kidneys.

Funder

South-Eastern Norway Regional Health Authority

Olav Raagholt og Gerd Meidel Raagholts

Nasjonalforeningen for folkehelsen

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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