MicroRNA miR171b Positively Regulates Resistance to Huanglongbing of Citrus
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Published:2023-03-17
Issue:6
Volume:24
Page:5737
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Lv Yuanda12, Zhong Yun12ORCID, Jiang Bo13, Yan Huaxue12, Ren Shuang1, Cheng Chunzhen1ORCID
Affiliation:
1. Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China 2. Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, China 3. Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangzhou 510640, China
Abstract
Huanglongbing (HLB) is one of the most severe citrus diseases in the world, causing huge economic losses. However, efficient methods of protecting citrus from HLB have not yet been developed. microRNA (miRNA)-mediated regulation of gene expression is a useful tool to control plant diseases, but the miRNAs involved in regulating resistance to HLB have not yet been identified. In this study, we found that miR171b positively regulated resistance to HLB in citrus. Upon infection with HLB bacteria, the bacteria were detected in the second month in the control plants. However, in the miR171b-overexpressing transgenic citrus plants, the bacteria could not be detected until the 24th month. RNA-seq data indicated that multiple pathways, such as photosynthesis, plant–pathogen interaction, the MAPK signaling pathway, etc., might be involved in improving the resistance to HLB in miR171b-overexpressing plants compared with the control. Finally, we determined that miR171b could target SCARECROW-like (SCL) genes to downregulate their expression, which then led to promoted resistance to HLB stress. Collectively, our results demonstrate that miR171b plays a positive regulatory role in resistance to citrus HLB, and provides a new insight into the role of miRNAs in the adaptation of citrus to HLB stress.
Funder
National Natural Science Foundation of China Special Fund for Scientific Innovation Strategy, Construction of High Level, Academy of Agriculture Science Guangdong Provincial Rural Revitalization Strategy Project open competition program of the top ten critical priorities of Agricultural Science and Technology Innovation for the 14th Five-year Plan of Guangdong Province Guangdong Provincial science and technology special project in 2021
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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