Affiliation:
1. State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, China
2. College of Life Science, Northeast Agricultural University, Harbin 150030, China
Abstract
Myostatin (MSTN) is a negative regulator of skeletal muscle genesis during development. MSTN mutation leads to increased lean meat production and reduced fat deposition in livestock. However, the mechanism by which MSTN promotes myogenesis by regulating metabolism is not clear. In this study, we compared the metabolomics of the livers of wild-type (WT) and MSTN mutation cattle (MT), and found changes in the content and proportion of fatty acids and bile acids in MT cattle. The differential metabolites were enriched in sterol synthesis and primary bile acid synthesis. We further analyzed the expression of genes involved in the regulation of lipid and bile acid metabolism, and found that the loss of MSTN may alter lipid synthesis and bile acid metabolism. This study provides new basic data for MSTN mutations in beef cattle breeding.
Funder
Inner Mongolia Autonomous Region Open Competition Projects
Inner Mongolia Autonomous Region Science and Technology Major Project
Inner Mongolia Autonomous Region Science and Technology Leading Team
Inner Mongolia Autonomous Region Young Talents Support Project
Central Government Guides Local Science and Technology Development Funds
Inner Mongolia Autonomous Region High-Level Talent Support Program
Inner Mongolia University Chief Scientist Program
Subject
Molecular Biology,Biochemistry,Endocrinology, Diabetes and Metabolism
Reference63 articles.
1. Regulation of skeletal muscle mass in mice by a new TGF-p superfamily member;McPherron;Nature,1997
2. Double muscling in cattle due to mutations in the myostatin gene;McPherron;Proc. Natl. Acad. Sci. USA,1997
3. Analysis of meat color, meat tenderness and fatty acid composition of meat in second filial hybrid offspring of MSTN mutant pigs;Gao;Meat Sci.,2022
4. Budsuren, U., Ulaangerel, T., Shen, Y., Liu, G., Davshilt, T., Yi, M., Bold, D., Zhang, X., Bai, D., and Dorjgotov, D. (2022). MSTN Regulatory Network in Mongolian Horse Muscle Satellite Cells Revealed with miRNA Interference Technologies. Genes, 13.
5. Expression of calpastatin and myostatin genes associated with lamb meat quality;Bagatoli;Genet. Mol. Res. GMR,2013