The Antifungal Properties of Super-Hydrophobic Nanoparticles and Essential Oils on Different Material Surfaces

Abstract

This study was undertaken to determine the in vitro antifungal activities of super-hydrophobic nanoparticles (SHNPs), essential oils (EOs), and their mixtures (SHNPs/EOs). We have applied a thin layer of SHNPs in combination with various concentrations of three EOs: Arborvitae (Thuja plicata), Oregano (Origanum vulgare L.), and Thyme (Thymus vulgaris). The mixtures were spread on the surface of different materials: whitewood, sandstone, and paper. The antifungal and protective properties of these SHNP and EO mixtures were evaluated. The parameter Rr (ratio of reflectivity) was determined to identify the color changes of substrates. Digital microscopy was used to measure the colonization area of molds and also their penetration in the analyzed materials. Surprisingly, the use of SHNPs alone showed a balanced compromise in order to inhibit the mold growth on assayed surfaces.