Non-Destructive Monitoring of P. fluorescens and S. epidermidis Biofilm under Different Media by Fourier Transform Infrared Spectroscopy and Other Corroborative Techniques

Abstract

In the present study, the early stage of bacteria biofilm formation has been studied as a function of different nutrients. Infrared spectra of Pseudomonas fluorescens (PF) and Staphylococcus epidermidis (SE), on germanium ATR crystal, were collected under deionized water H2O, phosphate buffered solution (PBS) and PBS with glucose (PBS-G). In H2O, protein bands of PF increased while, no difference in PBS and PBS-G were observed until 135 min. SE strain showed a low sensitivity to PBS composition starting to expose proteins on surfaces after 120 min. SE shows a low polysaccharides increase in H2O while, in bare and enriched PBS their intensity increases after 120 and 75 min. in PBS and PBS-G respectively. PF exhibits a peculiar behavior in H2O where the saccharide bands increased strongly after 100 min, while under all the other conditions, the intensity of polysaccharide bands increased up to the plateau probably because the layer of the biofilm exceeded the penetration capability of FTIR technique. All data suggest that, under lack of nutrients, both the bacteria tend to firmly anchor themselves to the support using proteins.