Oestrogen and Vibration Improve Intervertebral Disc Cell Viability and Decrease Catabolism in Bovine Organ Cultures

Author:

Widmayer Franziska1,Neidlinger-Wilke Cornelia1,Witz Fiona1,Jansen Jan U.1,Ignatius Anita1ORCID,Haffner-Luntzer Melanie1ORCID,Teixeira Graciosa Q.1ORCID

Affiliation:

1. Institute of Orthopaedic Research and Biomechanics, University of Ulm, 89081 Ulm, Germany

Abstract

Postmenopausal women are at an increased risk for intervertebral disc degeneration, possibly due to the decrease in oestrogen levels. Low-magnitude, high-frequency vibration (LMHFV) is applied as a therapeutic approach for postmenopausal osteoporosis; however, less is known regarding possible effects on the intervertebral disc (IVD) and whether these may be oestrogen-dependent. The present study investigated the effect of 17β-oestradiol (E2) and LMHFV in an IVD organ culture model. Bovine IVDs (n = 6 IVDs/group) were treated with either (i) E2, (ii) LMHFV or (iii) the combination of E2 + LMHFV for 2 or 14 days. Minor changes in gene expression, cellularity and matrix metabolism were observed after E2 treatment, except for a significant increase in matrix metalloproteinase (MMP)-3 and interleukin (IL)-6 production. Interestingly, LMHFV alone induced cell loss and increased IL-6 production compared to the control. The combination of E2 + LMHFV induced a protective effect against cell loss and decreased IL-6 production compared to the LMHFV group. This indicates possible benefits of oestrogen therapy for the IVDs of postmenopausal women undergoing LMHFV exercises.

Funder

Financial Support Programmes for Female Researchers, Office for Gender Equality, Ulm University

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

Reference62 articles.

1. GBD 2015 Disease and Injury Incidence and Prevalence Collaborators (2016). Global, regional, and national incidence, prevalence, and years lived with disability for 310 diseases and injuries, 1990–2015: A systematic analysis for the Global Burden of Disease Study 2015. Lancet, 388, 1545–1602.

2. Global, regional, and national disability-adjusted life years (DALYs) for 306 diseases and injuries and healthy life expectancy (HALE) for 188 countries, 1990–2013: Quantifying the epidemiological transition;Murray;Lancet,2015

3. A systematic review of the global prevalence of low back pain;Hoy;Arthritis Rheum.,2012

4. Prevalence and pattern of lumbar magnetic resonance imaging changes in a population study of one thousand forty-three individuals;Cheung;Spine,2009

5. Menopause is associated with lumbar disc degeneration: A review of 4230 intervertebral discs;Lou;Climacteric,2014

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