Effects of Chronic Heat Stress on Kidney Damage, Apoptosis, Inflammation, and Heat Shock Proteins of Siberian Sturgeon (Acipenser baerii)
Author:
Jing Zhaoxin1ORCID, Chen Qianyu1, Yan Chaozhan1, Zhang Chaoyang2, Xu Zihan1, Huang Xiaoli1, Wu Jiayun2, Li Yunkun2ORCID, Yang Shiyong1ORCID
Affiliation:
1. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China 2. College of Life Sciences, Sichuan Agricultural University, Ya’an 625000, China
Abstract
Chronic heat stress caused by global warming can have serious implications for fish survival. The kidney plays a central role in many homeostatic functions, including water and electrolyte regulation. However, there is limited knowledge about the effect of heat stress on fish kidneys. In this study, water temperatures were increased from 20 °C to 24 °C and 28 °C in 8 days at a warming rate of 1 °C/d, and then maintained for 12 days. We investigated the effects of mild heat stress (24 °C) and high heat stress (28 °C) on Siberian Sturgeon (Acipenser baerii) kidneys using histological observation, flow cytometry detection, and RT-qPCR. Our histological observations revealed that heat stress caused significant infiltration of inflammatory cells in the kidney, especially at 28 °C. The flow cytometry assay demonstrated a significant increase in the number of apoptotic cells after heat stress at 28 °C compared to a control group at 20 °C (p = 0.033). The level of plasma creatinine was significantly increased in the 28 °C group compared to the control group (p = 0.001). In addition, the mRNA expression levels of heat shock protein GRP75 increased (p = 0.009). The results indicate that heat stress at 28 °C caused damage to the kidneys of A. baerii and triggered the protective response of heat shock proteins. In conclusion, this study contributes to the understanding of the coping strategies of the kidney of A. baerii for chronic heat stress.
Funder
Natural Science Foundation of Sichuan Province of China Sichuan Science and Technology Program Project of Sichuan Innovation Team of National Modern Agricultural Industry Technology System Teaching Reform Project of Sichuan Province Science and Technology Achievement Transfer and Transformation Demonstration Project of Sichuan Province Youth Foundation of Natural Science Foundation of Sichuan Province
Subject
General Veterinary,Animal Science and Zoology
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