Integrated Transcriptome and Metabolome Analyses Provide Insights into the Coloring Mechanism of Dark-red and Yellow Fruits in Chinese Cherry [Cerasus pseudocerasus (Lindl.) G. Don]
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Published:2023-02-09
Issue:4
Volume:24
Page:3471
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Wang Yan12, Wang Zhiyi12, Zhang Jing12, Liu Zhenshan12, Wang Hao12, Tu Hongxia12, Zhou Jingting12, Luo Xirui1, Chen Qing1ORCID, He Wen12, Yang Shaofeng12, Li Mengyao1ORCID, Lin Yuanxiu12ORCID, Zhang Yunting12, Zhang Yong1ORCID, Luo Ya1, Tang Haoru12ORCID, Wang Xiaorong12ORCID
Affiliation:
1. College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China 2. Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, China
Abstract
Chinese cherry [Cerasus pseudocerasus (Lindl.) G. Don] is an important fruit tree from China that has excellent ornamental, economic, and nutritional values with various colors. The dark-red or red coloration of fruit, an attractive trait for consumers, is determined by anthocyanin pigmentation. In this study, the coloring patterns during fruit development in dark-red and yellow Chinese cherry fruits were firstly illustrated by integrated transcriptome and widely-targeted metabolome analyses. Anthocyanin accumulation in dark-red fruits was significantly higher compared with yellow fruits from the color conversion period, being positively correlated to the color ratio. Based on transcriptome analysis, eight structural genes (CpCHS, CpCHI, CpF3H, CpF3’H, CpDFR, CpANS, CpUFGT, and CpGST) were significantly upregulated in dark-red fruits from the color conversion period, especially CpANS, CpUFGT, and CpGST. On contrary, the expression level of CpLAR were considerably higher in yellow fruits than in dark-red fruits, especially at the early stage. Eight regulatory genes (CpMYB4, CpMYB10, CpMYB20, CpMYB306, bHLH1, CpNAC10, CpERF106, and CpbZIP4) were also identified as determinants of fruit color in Chinese cherry. Liquid chromatography-tandem mass spectrometry identified 33 and 3 differential expressed metabolites related to anthocyanins and procyanidins between mature dark-red and yellow fruits. Cyanidin-3-O-rutinoside was the predominant anthocyanin compound in both fruits, while it was 6.23-fold higher in dark-red than in yellow fruits. More accumulated flavanol and procyanidin contents resulted in less anthocyanin content in flavonoid pathway in yellow fruits due to the higher expression level of CpLAR. These findings can help understand the coloring mechanism of dark-red and yellow fruits in Chinese cherry, and provide genetic basis for breeding new cultivars.
Funder
Cherry Resources Sharing and Service Platform of Sichuan Province Tianfu Talent Project of Chengdu City Rural Revitalization Research Institute in Tianfu New Area of Sichuan Province Undergraduate Innovation Training Program in Sichuan Agricultural University Shuangzhi Project Innovation Team of Sichuan Agricultural University
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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