UPLC–MS/MS and Gene Expression Research to Distinguish the Colour Differences of Rhododendron liliiflorum H. Lév
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Published:2023-12-18
Issue:12
Volume:9
Page:1351
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ISSN:2311-7524
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Container-title:Horticulturae
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language:en
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Short-container-title:Horticulturae
Author:
Dai Jin1ORCID, Wang Xinglin1ORCID, Meng Xingpan1ORCID, Zhang Xu2, Zhou Qihang3ORCID, Zhang Zhengdong4, Zhang Ximin1ORCID, Yi Yin1, Liu Lunxian1ORCID, Shen Tie13ORCID
Affiliation:
1. Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China, Engineering Research Center of Carbon Neutrality in Karst Areas, Ministry of Education, Key Laboratory of Environment Friendly Management on High Altitude Rhododendron Diseases and Pests, Institutions of Higher Learning in Guizhou Province, School of Life Science, Guizhou Normal University, Guiyang 550025, China 2. Guizhou Caohai Wetland Ecosystem National Observation and Research Station, Guizhou Academy of Forestry Sciences, Guiyang 550001, China 3. Key Laboratory of Information and Computing Science Guizhou Province, School of Mathematical Sciences, Guizhou Normal University, Guizhou 550025, China 4. College of Mathematics and Information Science, Guiyang University, Guiyang 550001, China
Abstract
Among ornamental plants, the colour of the petals is an important feature. However, the reason for the colour differences of Rhododendron liliiflorum remains unclear. To reveal the differences in the colour of R. liliiflorum, high-efficiency liquid chromatographic collar (UPLC–MS/MS) technology was used to study the yellow and white parts of R. liliiflorum. A total of 1187 metabolites were identified in R. liliiflorum petals, including 339 flavonoid metabolites. Seventy-eight types of flavonoids in these metabolites were found in the yellow and white parts of R. liliiflorum petals, along with 11 other significantly enriched substances. Combining gene expression-related data with differential metabolite data demonstrated effects of enrichment in the flavanonols (fustin), flavonols (epiafzelechin and afzelechin), and flavanones (pinocembrin) of flavonoid biosynthesis; glyccitin, 6″-O-malonylgenistin, and 6-hydroxydaidzein of isoflavonoid biosynthesis; and anthocyanin biosynthesis of malvidin-3-O-galactoside (primulin), delphinidin-3-O-rutinoside, cyanidin-3-O-glucoside (kuromanin), and cyanidin-3-O-rutinoside (keracyanin), which are potentially the contributing factors responsible for the differences in petal colour in R. liliiflorum. This study establishes a connection between the differential metabolites underlying the color differences in the petals of R. liliiflorum and the gene expression in R. liliiflorum. This will provide a foundation for subsequent research on the regulation of flower color in R. liliiflorum and have profound implications for horticultural applications of R. liliiflorum.
Funder
Guizhou Provincial Science and Technology Projects Guizhou Provincial Basic Research Program National Science Foundation of China NSFC Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province, China Higher Education Science and Research Youth Project of Guizhou Education Department Key Laboratory of Environment Friendly Management on Alpine Rhododendron Diseases and Pests of Institutions of Higher Learning in Guizhou Province, Guizhou Normal University Research Foundation for Science & Technology Innovation Team of Guizhou Province
Subject
Horticulture,Plant Science
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