A New and Profitable Protocol to DNA Extraction in Limnospira maxima
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Published:2023-07-01
Issue:4
Volume:6
Page:62
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ISSN:2409-9279
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Container-title:Methods and Protocols
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language:en
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Short-container-title:MPs
Author:
Pineda-Rodriguez Yirlis Yadeth1, Pompelli Marcelo F.2ORCID, Jarma-Orozco Alfredo2ORCID, Rodríguez Novisel Veitía3, Rodriguez-Paez Luis Alfonso2ORCID
Affiliation:
1. Facultad de Ciencias Agrícolas, Universidad de Córdoba, Montería 230002, Colombia 2. Laboratorio de Biología Molecular Aplicada (INVEPAR) de la Facultad de Ciencias Agrícolas, Universidad de Córdoba, Montería 230002, Colombia 3. Instituto de Biotecnología de las Plantas, Universidad Central “Marta Abreu” de Las Villas, Santa Clara 54830, Cuba
Abstract
Limnospira maxima is a remarkable organism showing great potential as a versatile and sustainable food source, offering a powerful solution to address the pressing issues of malnutrition and undernourishment worldwide. L. maxima contains high amounts of proteins, vitamins, minerals, and essential fatty acids. It can be grown in both bioreactors and open systems; however, before considering industrial production, optimization studies of the cultivation must be conducted to obtain knowledge about the ideal environmental conditions. Additionally, for the molecular typing of L. maxima strains and their industrial scaling, high-quality and large quantity DNA extraction is required. Notwithstanding, DNA extraction from L. maxima can be challenging due to the low amount of DNA in cells and the presence of difficult-to-remove substances such as polysaccharides and polyphenols. In this study, the quality and quantity of DNA extracted from two types of L. maxima samples (Limnospira maxima strain SISCA accession GenBank: OR195505.1) were evaluated using three commercially available DNA extraction kits and two types of input biological material. The results showed that Pbact-P kit had the highest quantity and quality of DNA, while CTAB-P allowed for a higher quantity and quality of RNA, making them optimal protocols for nucleic acid extraction to improve PCR, rt-PCR, and genome sequencing of L. maxima compared with other extraction methods.
Subject
Biochemistry, Genetics and Molecular Biology (miscellaneous),Structural Biology,Biotechnology
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