Optimal Laboratory Cultivation Conditions of Limnospira maxima for Large-Scale Production
Author:
Pineda-Rodríguez Yirlis Yadeth1, Herazo-Cárdenas Diana Sofia2, Vallejo-Isaza Adriana2ORCID, Pompelli Marcelo F.3ORCID, Jarma-Orozco Alfredo3ORCID, Jaraba-Navas Juan de Dios3ORCID, Cordero-Ocampo Jhony David4, González-Berrio Marianella4, Arrieta Daniela Vegliante1, Pico-González Ana1, Ariza-González Anthony1ORCID, Aviña-Padilla Katia5ORCID, Rodríguez-Páez Luis Alfonso3
Affiliation:
1. Departamento de Ingeniería Agronómica y Desarrollo Rural, Maestría en Ciencias Agronómicas, Facultad de Ciencias Agrícolas, Universidad de Córdoba, Montería 230002, Colombia 2. Laboratorio de Sanidad Acuícola y Calidad de Agua, Facultad de Medicina Veterinaria y Zootecnia, Universidad de Córdoba, Montería 230002, Colombia 3. Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Agrícolas, Universidad de Córdoba, Montería 230002, Colombia 4. Departamento de Ciencias Acuícolas, Programa de Acuicultura, Facultad de Medicina Veterinaria y Zootecnia, Universidad de Córdoba, Montería 230002, Colombia 5. Centro de Investigación y de Estudios Avanzados del I.P.N. Unidad Irapuato, Irapuato 36821, Mexico
Abstract
Cultivating Limnospira maxima, renowned for its abundant proteins and valuable pigments, faces substantial challenges rooted in the limited understanding of its optimal growth parameters, associated high costs, and constraints in the procurement of traditional nitrogen sources, particularly NaNO3. To overcome these challenges, we conducted a comprehensive 4 × 3 factorial design study. Factors considered included white, red, blue, and yellow light spectra, along with nitrogen sources NaNO3 and KNO3, as well as a nitrogen-free control, for large-scale implementation. Optimal growth, measured by Optical Density, occurred with white and yellow light combined with KNO3 as the nitrogen source. These conditions also increased dry weight and Chl-a content. Cultures with nitrogen deprivation exhibited high values for these variables, attributed to carbon accumulation in response to nitrogen scarcity. Phycocyanin, a crucial pigment for nutrition and industry, reached its highest levels in cultures exposed to white light and supplemented with KNO3, with an impressive content of 384.11 g kg−1 of dry weight. These results highlight the efficacy and cost-efficiency of using a combination of white light and KNO3 for large-scale L. maxima cultivation. This strategy offers promising opportunities to address global food security challenges and enhance the production of industrially relevant pigments.
Funder
University of Córdoba
Subject
General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology
Reference47 articles.
1. Contribution of Small-scale Farmers to Global Food Security: A Meta-analysis;Azadi;J. Sci. Food Agric.,2023 2. FAO, FIDA, OMS, PMA, and UNICEF (2022). El Estado de la Seguridad Alimentaria y la Nutrición en el Mundo 2022, UNICEF. 3. Ravindran, B., Gupta, S., Cho, W.-M., Kim, J., Lee, S., Jeong, K.-H., Lee, D., and Choi, H.-C. (2016). Microalgae Potential and Multiple Roles—Current Progress and Future Prospects—An Overview. Sustainability, 8. 4. Tackling Community Undernutrition at Lake Bogoria, Kenya: The Potential of Spirulina (Arthrospira Fusiformis) as a Food Supplement;Yin;Afr. J. Food Agric. Nutr. Dev.,2017 5. Spirulina—From Growth to Nutritional Product: A Review;Soni;Trends Food Sci. Technol.,2017
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