Affiliation:
1. Institute of Food Science, Wenzhou Academy of Agricultural Science, Wenzhou 325006, China
2. Southern Zhejiang Key Laboratory of Crop Breeding, Wenzhou 325006, China
3. College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China
4. College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China
Abstract
Chlorine dioxide (ClO2) is widely used for the quality preservation of postharvest horticultural plants. However, the molecular mechanism of how ClO2 works is not clear. The purpose of this study was to understand ethylene-related molecular signaling in ClO2-treated fresh-cut cauliflower florets. Transcriptome analysis was used to investigate ethylene-related gene regulation. A total of 182.83 Gb clean data were acquired, and the reads of each sample to the unique mapped position of the reference genome could reach more than 85.51%. A sum of 2875, 3500, 4582 and 1906 differential expressed genes (DEGs) were identified at 0 d, 4 d, 8 d and 16 d between the control group and ClO2-treated group, respectively. DEGs were enriched in functions such as ‘response to oxygen-containing compounds’ and ‘phosphorylation’, as well as MAPK signaling pathway, plant hormone transduction pathway and so on. Genes, including OXI1, MPK3, WRKY22 and ERF1, which are located at the junction of wounding, pathogen attack, pathogen infection or ethylene signal transduction pathways, were up-regulated in response to stress. ETR and CTR1 (both up-regulated), as well as three down-regulated genes, including BolC5t34953H (a probable NAC), BolC1t05767H (a probable NAC) and BolC2t06548H (a probable ERF13), might work as negative regulators for ethylene signal transduction. In conclusion, ethylene-related genes and pathways are involved in ClO2 treatment, which might enhance stress resistance and have a negative feedback mechanism.
Funder
Public Welfare Technology Application Research Project of Zhejiang Province
Major scientific and technological project of Wenzhou