Bio-Assay-Guided Isolation of Fractions and Constituents with Antioxidant and Lipid-lowering Activity from Allium cepa

Author:

Fan Kongming12,Li Yan1,Fu Qiaofeng1,Wang Jinmin1,Lin Yong3,Qiu Linyu1,Ran Li1,Yang Junli4,Yang Chunyan1ORCID

Affiliation:

1. School of Pharmacy, Institute of Material Medica, North Sichuan Medical College, Nanchong 637100, China

2. Innovative Platform of Basic Medical Sciences, School of Basic Medical Sciences, North Sichuan Medical College, Nanchong 637100, China

3. School of Public Health, North Sichuan Medical College, Nanchong 637100, China

4. Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China

Abstract

Active fractions and constituents with antioxidant and lipid-lowering activities were investigated using bio-assay-guided isolation and identification. The data showed that the antioxidant fraction of A. cepa was AC50%, the main constituents of which were quercetin and isoquercitrin, by way of both ultra-high performance liquid chromatography–mass spectrometry (UPLC-MS) and bio-assay-guided purification and elucidation. Similarly, the lipid-lowering active fraction of A. cepa was AC30% with the main constituents of 3,4-dihydroxybenzoic acid and quercetin 3,4′-O-diglucoside. Also, bio-assay-guided isolation led to the isolation and identification of five known compounds with a purity of more than 98%, and quercetin was both the best free radical scavenger and lipid-lowering constituent. Moreover, the mechanism of the lipid-lowering effect of AC30% might be its reduction in mRNA expression levels of sterol regulatory element binding protein 2 (SREBP2) and FAS gene in lipid synthesis. Otherwise, reducing the mRNA expression level of lipid synthesis genes, including SREBP1, SREBP2, fatty acid synthetase (FASN), β-Hydroxy β-methylglutaryl-CoA (HMGCR), stearoyl CoA desaturase 1 (SCD1), and increasing the mRNA expression level of lipid decomposition gene, such as carnitine palmitoyl transferease-1 (CPT1), might be involved in the lipid-lowering activity of quercetin. This study suggested that Allium cepa might be used to prevent and treat oxidative stress and dislipidemia-related disorders, including NAFLD.

Funder

Sichuan college students’ innovation training project

Sichuan Provincial Education Department

North Sichuan Medical College

Sichuan Provincial Science and Technology Department

CAS Key Laboratory of Chemistry of Northwestern Plant Resources

Publisher

MDPI AG

Subject

Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology

Reference40 articles.

1. Effects of Onion Alcohol Extract on the Function of Isolated Toad Heart;Lv;West. J. Tradit. Chin. Med.,2018

2. Bioactive components of onion (Allium cepa L.)—A Review;Acta Aliment.,2013

3. Onion and garlic for prevention of atherosclerosis;Zhao;J. Environ. Hyg.,1979

4. The Effects of Onion Consumption on Prevention of Nonalcoholic Fatty Liver Disease;Emamat;Indian J. Clin. Biochem.,2018

5. Flavonoids: An overview;Panche;J. Nutr. Sci.,2016

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3